Detailed information NTG0888255

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RUW23_RS10645
• Genome accession: NZ_CP136014
• Coordinates: 2254852..2256000 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain R18-20
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2249852..2261000

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RUW23_RS10625 (RUW23_10625)	-	2250589..2251773 (-)	1185	WP_003100953.1	cystathionine gamma-synthase	-
RUW23_RS10630 (RUW23_10630)	-	2251770..2253143 (-)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
RUW23_RS10635 (RUW23_10635)	-	2253386..2253790 (-)	405	WP_003084560.1	NINE protein	-
RUW23_RS10640 (RUW23_10640)	czcD	2253945..2254844 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
RUW23_RS10645 (RUW23_10645)	pilU	2254852..2256000 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
RUW23_RS10650 (RUW23_10650)	pilT	2256178..2257212 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
RUW23_RS10655 (RUW23_10655)	-	2257427..2258119 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
RUW23_RS10660 (RUW23_10660)	proC	2258131..2258952 (+)	822	WP_031757004.1	pyrroline-5-carboxylate reductase	-
RUW23_RS10665 (RUW23_10665)	-	2258963..2259556 (+)	594	WP_003084544.1	YggT family protein	-
RUW23_RS10670 (RUW23_10670)	-	2259553..2259849 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=888255 RUW23_RS10645 WP_003100959.1 2254852..2256000(-) (pilU) [Pseudomonas aeruginosa strain R18-20]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=888255 RUW23_RS10645 WP_003100959.1 2254852..2256000(-) (pilU) [Pseudomonas aeruginosa strain R18-20]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382