Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RRX38_RS02535 Genome accession   NZ_CP135971
Coordinates   594327..595502 (+) Length   391 a.a.
NCBI ID   WP_315961378.1    Uniprot ID   A0AA96U258
Organism   Pseudomonas sp. DTU_2021_1001937_2_SI_NGA_ILE_001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 589327..600502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RRX38_RS02495 (RRX38_02495) - 589368..589955 (+) 588 WP_315961370.1 hypothetical protein -
  RRX38_RS02500 (RRX38_02500) comJ 590061..590540 (-) 480 WP_315961371.1 competence protein ComJ -
  RRX38_RS02505 (RRX38_02505) - 591013..591798 (+) 786 WP_315961372.1 SDR family NAD(P)-dependent oxidoreductase -
  RRX38_RS02510 (RRX38_02510) - 591931..592236 (+) 306 WP_315961373.1 hypothetical protein -
  RRX38_RS02515 (RRX38_02515) - 592274..592549 (-) 276 WP_315961374.1 hypothetical protein -
  RRX38_RS02520 (RRX38_02520) - 593104..593544 (+) 441 WP_315961375.1 hemerythrin domain-containing protein -
  RRX38_RS02525 (RRX38_02525) - 593598..593945 (+) 348 WP_315961376.1 2-oxoacid ferredoxin oxidoreductase -
  RRX38_RS02530 (RRX38_02530) - 593963..594238 (-) 276 WP_315961377.1 peptidylprolyl isomerase -
  RRX38_RS02535 (RRX38_02535) pilU 594327..595502 (+) 1176 WP_315961378.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RRX38_RS02540 (RRX38_02540) - 595523..597100 (-) 1578 WP_315961379.1 ABC transporter ATP-binding protein -
  RRX38_RS02545 (RRX38_02545) - 597102..598124 (-) 1023 WP_315961380.1 ABC transporter permease -
  RRX38_RS02550 (RRX38_02550) - 598124..599188 (-) 1065 WP_315961381.1 microcin C ABC transporter permease YejB -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 43033.17 Da        Isoelectric Point: 6.5093

>NTDB_id=887952 RRX38_RS02535 WP_315961378.1 594327..595502(+) (pilU) [Pseudomonas sp. DTU_2021_1001937_2_SI_NGA_ILE_001]
MDFQALLKTLATQDGSDLYLSTGAPPCAKFNGVLRPLGSETLKPGDVARVAEAIMDEEQRRVFDRELEMNLAVSVAGVGR
FRINIFMQRNEVSIVARNIKLDIPKFEDLHLPPVLLDVVMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGSPTIRDLIQRNELTEIKGIMEKSENLGMQTFDTAL
YRLVVEGAISEEEAIKHADSKNNLRLRLKLHGEGGVTAAPTQAPAAPVGVASPSMAQWGLVDDDEQGPGQA

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=887952 RRX38_RS02535 WP_315961378.1 594327..595502(+) (pilU) [Pseudomonas sp. DTU_2021_1001937_2_SI_NGA_ILE_001]
ATGGATTTCCAGGCATTACTCAAAACCCTGGCCACCCAGGACGGTTCGGACCTCTATCTATCCACCGGCGCGCCACCCTG
CGCCAAGTTCAATGGCGTGCTGCGCCCGCTGGGCAGCGAAACCCTCAAGCCCGGTGATGTGGCGCGGGTGGCCGAGGCGA
TCATGGACGAAGAGCAGCGGCGCGTCTTCGACCGCGAGCTGGAGATGAACCTGGCGGTGTCGGTGGCCGGGGTCGGGCGC
TTCCGTATCAACATCTTCATGCAGCGCAACGAAGTGTCGATCGTGGCGCGCAACATCAAGCTGGACATTCCCAAGTTCGA
AGACCTGCATCTGCCGCCGGTGCTGCTCGACGTGGTGATGGAAAAGCACGGTCTGGTGCTGTTCGTGGGCGCCACCGGTT
CGGGCAAGTCCACCTCCCTGGCGGCACTGATCGACTACCGCAACCGTAACGCCAGCGGGCACATCATCACCATCGAAGAC
CCGGTGGAGTTCATTCACCGGCACAAGAAGTCGATCATCAACCAGCGCGAGGTGGGCGTGGACACCCGCAGCTTTCATGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATTGGCGAGATCCGCGACCGCGAAACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGGCACCTGGCGATCTCGACCCTGCATGCCAACAACGCCAACCAGGCGCTGGACCGAATC
ATCAACTTCTTCCCTGAAGAACGCCGCGCCCAGTTGCTGCACGACCTGGGCAACAACCTCAAGGCCTTCGTTTCCCAGCG
CCTGGTGCGCACGCCCGACGGCAAGCGTCGCGCGGCGGTGGAGGTGATGATGGGCTCGCCGACCATCCGTGACCTGATCC
AGCGCAACGAGCTGACCGAAATCAAGGGCATCATGGAGAAGTCCGAGAACCTCGGCATGCAGACCTTCGACACCGCGCTG
TACCGCCTGGTGGTGGAGGGGGCGATCAGCGAGGAAGAGGCCATCAAGCATGCCGACTCGAAGAACAACTTGCGCCTGCG
CCTGAAGCTGCATGGTGAAGGCGGCGTGACCGCTGCGCCCACCCAGGCGCCCGCCGCGCCGGTGGGCGTGGCCAGCCCGA
GTATGGCGCAGTGGGGGCTGGTGGACGACGATGAGCAAGGGCCCGGGCAGGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.627

90.537

0.522
  pilU Acinetobacter baylyi ADP1

52.101

91.304

0.476
  pilU Vibrio cholerae strain A1552

53.143

89.514

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.791

97.698

0.389
  pilT Pseudomonas aeruginosa PAK

41.57

87.98

0.366
  pilT Acinetobacter baumannii strain A118

42.216

85.422

0.361
  pilT Acinetobacter nosocomialis M2

42.216

85.422

0.361
  pilT Acinetobacter baumannii D1279779

42.216

85.422

0.361