Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   RRU92_RS01960 Genome accession   NZ_CP135436
Coordinates   389632..390930 (-) Length   432 a.a.
NCBI ID   WP_281335419.1    Uniprot ID   A0AA96MNE6
Organism   Streptococcus sp. DTU_2020_1001019_1_SI_AUS_MUR_006     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 384632..395930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RRU92_RS01940 (RRU92_01940) rplI 385780..386232 (-) 453 WP_000864205.1 50S ribosomal protein L9 -
  RRU92_RS01945 (RRU92_01945) - 386229..388202 (-) 1974 WP_315640203.1 DHH family phosphoesterase -
  RRU92_RS01950 (RRU92_01950) hpf 388351..388893 (-) 543 WP_045762429.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  RRU92_RS01955 (RRU92_01955) comFC/cflB 388973..389635 (-) 663 WP_315640204.1 ComF family protein Machinery gene
  RRU92_RS01960 (RRU92_01960) comFA/cflA 389632..390930 (-) 1299 WP_281335419.1 DEAD/DEAH box helicase Machinery gene
  RRU92_RS01965 (RRU92_01965) - 390986..391621 (+) 636 WP_315640205.1 YigZ family protein -
  RRU92_RS01970 (RRU92_01970) - 391638..392081 (+) 444 WP_281335421.1 PH domain-containing protein -
  RRU92_RS01975 (RRU92_01975) cysK 392178..393104 (+) 927 WP_315640206.1 cysteine synthase A -
  RRU92_RS01980 (RRU92_01980) tsf 393229..394269 (-) 1041 WP_000808094.1 translation elongation factor Ts -
  RRU92_RS01985 (RRU92_01985) rpsB 394348..395130 (-) 783 WP_000268462.1 30S ribosomal protein S2 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49594.36 Da        Isoelectric Point: 8.6056

>NTDB_id=886509 RRU92_RS01960 WP_281335419.1 389632..390930(-) (comFA/cflA) [Streptococcus sp. DTU_2020_1001019_1_SI_AUS_MUR_006]
MKVNPNYLGRLFTEQELSHEERQQAKQIPSMKKEKDQQYCQRCGSQIEEEWHLPIGAFYCRECLIMKRIRSDQNLYYFPQ
ERFPQQDVLKWSGQLTPFQERVSQGLLQAVDKKEATLVHAVTGAGKTEMIYQVVAKVIDQGGAVCLASPRIDVCLELHKR
LQNDFSCEIALLYGESDPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPVLYHAVNNCVKETGLRIFLTATST
DELDRKVKQGELKRLSLPRRFHGNPLIVPKPIWLSNFNKYIEKNGLPPKLKLYIEKQRKTAYPLLIFASEIKKGEKLKEI
LQEKFPNEKIGFVSSITADRLEQVQAFRDGDLTILISTTILERGVTFPRVDVFVVEANHQLFTKSSLVQIGGRVGRSMDR
PTGELIFFHDGLNLSIKKAIKEIKQMNQEAGV

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=886509 RRU92_RS01960 WP_281335419.1 389632..390930(-) (comFA/cflA) [Streptococcus sp. DTU_2020_1001019_1_SI_AUS_MUR_006]
ATGAAAGTAAATCCAAATTATCTCGGTCGCCTTTTTACCGAGCAAGAATTAAGTCATGAGGAGAGACAACAAGCAAAACA
AATTCCATCCATGAAAAAAGAAAAGGATCAACAATATTGTCAGCGTTGTGGTAGTCAGATAGAAGAAGAATGGCATTTGC
CTATTGGTGCTTTTTATTGCCGAGAATGCTTGATTATGAAGCGAATAAGAAGTGATCAAAATCTCTATTATTTTCCGCAA
GAAAGGTTTCCACAGCAAGATGTACTCAAATGGTCAGGTCAACTGACTCCATTTCAAGAGCGGGTATCACAAGGACTCCT
TCAGGCGGTAGATAAGAAGGAGGCTACCTTGGTACATGCAGTGACAGGAGCTGGTAAGACAGAGATGATCTACCAAGTTG
TCGCCAAGGTAATTGATCAAGGTGGAGCTGTTTGTTTGGCCAGTCCAAGGATAGATGTTTGCTTAGAGCTCCATAAACGA
TTACAAAATGATTTTTCATGTGAGATTGCACTATTATATGGGGAGTCGGATCCCTATTTTCGTACACCTTTAGTTGTAGC
AACCACTCACCAACTACTAAAATTTTATCAAGCTTTTGATTTATTGATTGTGGATGAAGTGGATGCCTTTCCTTATGTTG
ATAATCCAGTGCTTTACCATGCTGTCAATAATTGTGTAAAGGAGACCGGATTGAGAATATTTTTAACAGCAACGTCAACT
GATGAGTTAGATAGAAAAGTTAAACAAGGGGAGTTAAAGCGTCTCAGTTTACCTCGTCGTTTTCATGGAAATCCTCTCAT
TGTTCCCAAGCCAATCTGGTTATCAAATTTTAATAAATATATAGAAAAAAATGGTTTACCTCCAAAATTAAAACTTTACA
TCGAGAAACAGAGAAAGACGGCCTATCCCTTACTAATCTTTGCCTCTGAGATTAAAAAGGGAGAAAAGCTAAAAGAAATT
TTGCAGGAGAAGTTTCCAAATGAGAAAATAGGATTTGTATCTTCAATCACAGCAGACCGATTAGAGCAGGTACAAGCCTT
TCGAGATGGAGACTTAACGATATTAATAAGCACAACTATATTAGAGCGTGGAGTTACCTTCCCTCGTGTAGATGTTTTTG
TGGTGGAAGCCAATCATCAACTCTTTACCAAGTCAAGTTTAGTACAAATTGGTGGGCGAGTTGGAAGAAGCATGGATCGT
CCTACTGGAGAACTGATATTTTTCCATGACGGACTCAATCTGTCCATTAAGAAAGCCATAAAGGAGATTAAACAAATGAA
TCAGGAGGCAGGAGTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(294-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

83.796

100

0.838
  comFA/cflA Streptococcus pneumoniae Rx1

83.565

100

0.836
  comFA/cflA Streptococcus pneumoniae D39

83.565

100

0.836
  comFA/cflA Streptococcus pneumoniae R6

83.565

100

0.836
  comFA/cflA Streptococcus pneumoniae TIGR4

83.333

100

0.833
  comFA/cflA Streptococcus mitis SK321

83.102

100

0.831
  comFA Lactococcus lactis subsp. cremoris KW2

53.553

91.204

0.488
  comFA Latilactobacillus sakei subsp. sakei 23K

38.051

99.769

0.38
  comFA Bacillus subtilis subsp. subtilis str. 168

38.78

94.907

0.368