Detailed information NTG0886420

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: RRP28_RS02460
• Genome accession: NZ_CP135285
• Coordinates: 537487..538551 (+)
• Length: 354 a.a.
• NCBI ID: WP_003378852.1
• Uniprot ID: A0A2G9KZJ9
• Organism: Pseudomonas syringae pv. actinidiae strain Yunnan2.4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 532487..543551

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RRP28_RS02450 (RRP28_02450)	-	533242..534510 (+)	1269	WP_003378855.1	malic enzyme-like NAD(P)-binding protein	-
RRP28_RS02455 (RRP28_02455)	-	534836..537277 (-)	2442	WP_370445385.1	penicillin-binding protein 1A	-
RRP28_RS02460 (RRP28_02460)	pilM	537487..538551 (+)	1065	WP_003378852.1	pilus assembly protein PilM	Machinery gene
RRP28_RS02465 (RRP28_02465)	-	538551..539177 (+)	627	WP_003378851.1	PilN domain-containing protein	-
RRP28_RS02470 (RRP28_02470)	pilO	539174..539797 (+)	624	WP_003378850.1	type 4a pilus biogenesis protein PilO	-
RRP28_RS02475 (RRP28_02475)	pilP	539794..540321 (+)	528	WP_003378849.1	type 4a pilus biogenesis lipoprotein PilP	-
RRP28_RS02480 (RRP28_02480)	pilQ	540369..542474 (+)	2106	WP_017682642.1	type IV pilus secretin PilQ	Machinery gene
RRP28_RS02485 (RRP28_02485)	aroK	542479..542997 (+)	519	WP_003378847.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38103.62 Da
• Isoelectric Point: 4.7008

>NTDB_id=886420 RRP28_RS02460 WP_003378852.1 537487..538551(+) (pilM) [Pseudomonas syringae pv. actinidiae strain Yunnan2.4]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTNVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=886420 RRP28_RS02460 WP_003378852.1 537487..538551(+) (pilM) [Pseudomonas syringae pv. actinidiae strain Yunnan2.4]
GTGTTCGAACTCTTTGGCAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCGTTACCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGTTGTCTCGCGTACTGGTCAAGGCCAAGACCAACGTCAAGATTGTGGCGGTT
GCCGTGGCGGGTTCCGCTGTGATCACCAAAACCATCGAGATGGACGCCGGTCTCTCCGACGACGACATGGAAAATCAGCT
TAAGCTTGAAGCTGATCAGTACATCCCTTATCCGCTCGAAGAAGTTGCCATCGATTTTGAAGTGCAGGGCTACTCGGTCC
GCAACCCGGAACGTGTCGAAGTCCTGTTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTGCGCGAGGCTGCGCTGGCTCTC
GCCGGGCTGACGGCGCGTGTCGTGGACGTCGAGGCCTACGCGCTGGAGCGTTCATTCGGGCTTCTGGCTGCGCAACTGGG
CAACGGTCATGACGAACTGACCGTTGCCGTGGTAGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACCACGGCC
GAATCATCTATACCCGCGAGCAATTGTTCGGCGGCCGCCAGCTGACTGACGAAATTCAGCGTCGCTACGGGCTTTCCATG
GAAGAGGCCGGGCTGGCCAAGAAGCAGGGCGGGCTGCCTGACGATTATGTCAGCGAAGTGCTGGACCCCTTCAAGGACGC
GCTCGTGCAACAGGTTTCCCGATCCCTGCAGTTCTTCTTTGCGGCCGGTCAGTACAACTCCGTGGATCACATCATGCTGG
CAGGCGGTACGGCTTCGATTTCAGGGCTGGAGCATTTGATTCAGCGACGTATCGGCACGCCGACCATGGTTGCCAACCCG
TTTGCCGACATGGCCTTGAGCTCCAAGGTCAATGCCGGTGCGCTGGCCAGCGATGCGCCTGCCCTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A2G9KZJ9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469