Detailed information NTG0885888

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ROT04_RS01975
• Genome accession: NZ_CP135174
• Coordinates: 419102..420250 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain PARM_L1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 414102..425250

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ROT04_RS01950 (ROT04_01950)	-	415253..415549 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ROT04_RS01955 (ROT04_01955)	-	415546..416139 (-)	594	WP_003084544.1	YggT family protein	-
ROT04_RS01960 (ROT04_01960)	proC	416150..416971 (-)	822	WP_128733698.1	pyrroline-5-carboxylate reductase	-
ROT04_RS01965 (ROT04_01965)	-	416983..417675 (-)	693	WP_128733697.1	YggS family pyridoxal phosphate-dependent enzyme	-
ROT04_RS01970 (ROT04_01970)	pilT	417890..418924 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ROT04_RS01975 (ROT04_01975)	pilU	419102..420250 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
ROT04_RS01980 (ROT04_01980)	czcD	420258..421157 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ROT04_RS01985 (ROT04_01985)	-	421311..421715 (+)	405	WP_003084560.1	NINE protein	-
ROT04_RS01990 (ROT04_01990)	-	421958..423331 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
ROT04_RS01995 (ROT04_01995)	-	423328..424512 (+)	1185	WP_128733696.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=885888 ROT04_RS01975 WP_003110016.1 419102..420250(+) (pilU) [Pseudomonas aeruginosa strain PARM_L1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=885888 ROT04_RS01975 WP_003110016.1 419102..420250(+) (pilU) [Pseudomonas aeruginosa strain PARM_L1]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382