Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RQL82_RS13750 Genome accession   NZ_CP135159
Coordinates   2916150..2917187 (+) Length   345 a.a.
NCBI ID   WP_262441968.1    Uniprot ID   -
Organism   Acinetobacter pittii strain AR3651     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2911150..2922187
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RQL82_RS13740 (RQL82_13800) sbcC 2911534..2915130 (+) 3597 WP_329759704.1 AAA family ATPase -
  RQL82_RS13745 (RQL82_13805) yggS 2915331..2916023 (-) 693 WP_329759705.1 YggS family pyridoxal phosphate-dependent enzyme -
  RQL82_RS13750 (RQL82_13810) pilT 2916150..2917187 (+) 1038 WP_262441968.1 type IV pilus twitching motility protein PilT Machinery gene
  RQL82_RS13755 (RQL82_13815) pilU 2917207..2918325 (+) 1119 WP_005070855.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RQL82_RS13760 (RQL82_13820) fur 2918395..2918832 (-) 438 WP_016141990.1 ferric iron uptake transcriptional regulator -
  RQL82_RS13765 (RQL82_13825) omlA 2918944..2919342 (+) 399 WP_002121740.1 outer membrane protein assembly factor BamE -
  RQL82_RS13770 (RQL82_13830) - 2919407..2919721 (-) 315 WP_329759707.1 RnfH family protein -
  RQL82_RS13775 (RQL82_13835) - 2919721..2920788 (-) 1068 WP_329759708.1 hypothetical protein -
  RQL82_RS13780 (RQL82_13840) - 2920942..2921397 (-) 456 WP_002121698.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38375.00 Da        Isoelectric Point: 6.7269

>NTDB_id=885744 RQL82_RS13750 WP_262441968.1 2916150..2917187(+) (pilT) [Acinetobacter pittii strain AR3651]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALDHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMMDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KNLVSRGVISPLTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=885744 RQL82_RS13750 WP_262441968.1 2916150..2917187(+) (pilT) [Acinetobacter pittii strain AR3651]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACACCTATCTGCTGGCATGCCACCAAT
GATTCGCGTTGATGGTGAAGTCCGTCGCATTAACTTACCTGCTCTAGATCATAAAGATGTTCATCGTTTGGTCTACGACA
TCATGAATGATAAACAGCGTCGTGACTACGAAGAAAAACTCGAAACCGACTTTTCTTTTGAAGTGCCCAACGTTGCTCGT
TTCCGTGTAAACGCATTTAACCAAAACCGTGGTGCGGGTGCAGTGTTTCGTACCATTCCGTCCAAAGTACTCACCATGGA
AGATTTAGGCTTAGGACAAATCTTTAAAGACATTTGTGACTATCCTCGCGGGATTGTACTGGTGACAGGTCCAACAGGTT
CGGGTAAATCAACGACGCTTGCAGCGATGATGGACTACATCAACGAAAATCGTTATGACCACATTCTAACGGTTGAAGAC
CCTATCGAATTTGTACACCAATCTAAGAAATGTTTGATTAACCAACGTGAAGTGCATCGTGATACACACGGCTTTAATGA
GGCGCTACGTTCAGCACTGCGTGAAGACCCAGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAGACTATTCGTTTAG
CGCTTACCGCTGCCGAAACAGGTCATTTGGTGTTTGGCACGCTGCATACCACTTCTGCTGCGAAAACCATTGACCGTGTA
ATTGACGTATTCCCAGCAGAAGAAAAAGACATGGTTCGTGCCATGCTGTCTGAATCTTTACAGGCTGTTATTTCACAAAC
CCTGCTTAAAAAGAATGGCGGTGGCCGTGTCGCAGCCCATGAAATCATGATTGGTATTCCTGCAATTCGTAACCTAATTC
GTGAAAACAAAGTCGCGCAAATGTATTCAGCTATTCAGACTGGCGCAAATCATGGCATGACCACCCTCGACCAAAGCTTA
AAAAATTTGGTGTCTCGCGGTGTGATCAGCCCTCTAACAGCTCGTACTGCTGCCAAACAGCCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

98.841

100

0.988
  pilT Acinetobacter baumannii D1279779

98.551

100

0.986
  pilT Acinetobacter baumannii strain A118

98.551

100

0.986
  pilT Acinetobacter baylyi ADP1

88.663

99.71

0.884
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Pseudomonas aeruginosa PAK

83.14

99.71

0.829
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.644

95.362

0.693
  pilT Vibrio cholerae strain A1552

72.644

95.362

0.693
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.088

95.942

0.394
  pilU Acinetobacter baylyi ADP1

39.017

100

0.391