Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RO838_RS06020 Genome accession   NZ_CP135134
Coordinates   1345949..1347079 (+) Length   376 a.a.
NCBI ID   WP_011097971.1    Uniprot ID   Q87CD3
Organism   Xylella fastidiosa strain CFBP8478     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1340949..1352079
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RO838_RS06005 (RO838_06005) - 1341217..1342041 (+) 825 WP_057683466.1 glycosyltransferase family 2 protein -
  RO838_RS06010 (RO838_06010) - 1342695..1343522 (-) 828 WP_200904625.1 glycosyltransferase family 2 protein -
  RO838_RS06015 (RO838_06015) pilT 1344749..1345783 (+) 1035 WP_004084948.1 type IV pilus twitching motility protein PilT Machinery gene
  RO838_RS06020 (RO838_06020) pilU 1345949..1347079 (+) 1131 WP_011097971.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RO838_RS06025 (RO838_06025) - 1347772..1348740 (-) 969 WP_057683464.1 YafY family protein -
  RO838_RS06030 (RO838_06030) - 1349589..1350398 (+) 810 WP_004087308.1 DUF481 domain-containing protein -
  RO838_RS06035 (RO838_06035) hemC 1350452..1351369 (-) 918 WP_004087310.1 hydroxymethylbilane synthase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42479.73 Da        Isoelectric Point: 6.5563

>NTDB_id=885520 RO838_RS06020 WP_011097971.1 1345949..1347079(+) (pilU) [Xylella fastidiosa strain CFBP8478]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHEKCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLMDLSLNLKGIIAQQLIPSADGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNHNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGIEIAEMS

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=885520 RO838_RS06020 WP_011097971.1 1345949..1347079(+) (pilU) [Xylella fastidiosa strain CFBP8478]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCACTGACTTCGGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCACAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGTGGCATCATCATCATCGTGGGTG
CTACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAAATGCATCATCACCCAACGCGAAATAGGTACCGATACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAAGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCCGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATGGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTGCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCACAATGATCTTGGAATGACGACATTC
GATCAAAGTCTAGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGGAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAATAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87CD3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.58

97.606

0.601
  pilU Acinetobacter baylyi ADP1

60.169

94.149

0.566
  pilU Vibrio cholerae strain A1552

50.843

94.681

0.481
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.484

96.809

0.402