Detailed information NTG0885235

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RPM50_RS22410
• Genome accession: NZ_CP135099
• Coordinates: 4885261..4886409 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain Paer3125
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4880261..4891409

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RPM50_RS22385 (RPM50_22385)	-	4881412..4881708 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
RPM50_RS22390 (RPM50_22390)	-	4881705..4882298 (-)	594	WP_003084544.1	YggT family protein	-
RPM50_RS22395 (RPM50_22395)	proC	4882309..4883130 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
RPM50_RS22400 (RPM50_22400)	-	4883142..4883834 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
RPM50_RS22405 (RPM50_22405)	pilT	4884049..4885083 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
RPM50_RS22410 (RPM50_22410)	pilU	4885261..4886409 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
RPM50_RS22415 (RPM50_22415)	czcD	4886417..4887316 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
RPM50_RS22420 (RPM50_22420)	-	4887471..4887875 (+)	405	WP_003084560.1	NINE protein	-
RPM50_RS22425 (RPM50_22425)	-	4888118..4889491 (+)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
RPM50_RS22430 (RPM50_22430)	-	4889488..4890672 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=885235 RPM50_RS22410 WP_003110016.1 4885261..4886409(+) (pilU) [Pseudomonas aeruginosa strain Paer3125]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=885235 RPM50_RS22410 WP_003110016.1 4885261..4886409(+) (pilU) [Pseudomonas aeruginosa strain Paer3125]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTACACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATAACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382