Detailed information NTG0885190

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RPM47_RS04110
• Genome accession: NZ_CP135098
• Coordinates: 868472..869620 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain Paer2090
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 863472..874620

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RPM47_RS04090 (RPM47_04090)	-	864209..865393 (-)	1185	WP_015649763.1	cystathionine gamma-synthase	-
RPM47_RS04095 (RPM47_04095)	-	865390..866763 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
RPM47_RS04100 (RPM47_04100)	-	867006..867410 (-)	405	WP_003084560.1	NINE protein	-
RPM47_RS04105 (RPM47_04105)	czcD	867565..868464 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
RPM47_RS04110 (RPM47_04110)	pilU	868472..869620 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
RPM47_RS04115 (RPM47_04115)	pilT	869798..870832 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
RPM47_RS04120 (RPM47_04120)	-	871047..871739 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
RPM47_RS04125 (RPM47_04125)	proC	871751..872572 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
RPM47_RS04130 (RPM47_04130)	-	872583..873176 (+)	594	WP_003155373.1	YggT family protein	-
RPM47_RS04135 (RPM47_04135)	-	873173..873469 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=885190 RPM47_RS04110 WP_003100959.1 868472..869620(-) (pilU) [Pseudomonas aeruginosa strain Paer2090]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=885190 RPM47_RS04110 WP_003100959.1 868472..869620(-) (pilU) [Pseudomonas aeruginosa strain Paer2090]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382