Detailed information NTG0885154

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RPM46_RS07090
• Genome accession: NZ_CP135096
• Coordinates: 1560174..1561322 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain Paer3554
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1555174..1566322

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RPM46_RS07065 (RPM46_07065)	-	1556325..1556621 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
RPM46_RS07070 (RPM46_07070)	-	1556618..1557211 (-)	594	WP_003155373.1	YggT family protein	-
RPM46_RS07075 (RPM46_07075)	proC	1557222..1558043 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
RPM46_RS07080 (RPM46_07080)	-	1558055..1558747 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
RPM46_RS07085 (RPM46_07085)	pilT	1558962..1559996 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
RPM46_RS07090 (RPM46_07090)	pilU	1560174..1561322 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
RPM46_RS07095 (RPM46_07095)	czcD	1561330..1562229 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
RPM46_RS07100 (RPM46_07100)	-	1562384..1562788 (+)	405	WP_003084560.1	NINE protein	-
RPM46_RS07105 (RPM46_07105)	-	1563031..1564404 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
RPM46_RS07110 (RPM46_07110)	-	1564401..1565585 (+)	1185	WP_015649763.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=885154 RPM46_RS07090 WP_003100959.1 1560174..1561322(+) (pilU) [Pseudomonas aeruginosa strain Paer3554]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=885154 RPM46_RS07090 WP_003100959.1 1560174..1561322(+) (pilU) [Pseudomonas aeruginosa strain Paer3554]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382