Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   RKE25_RS04575 Genome accession   NZ_CP134867
Coordinates   1036962..1038020 (+) Length   352 a.a.
NCBI ID   WP_311841080.1    Uniprot ID   A0AA96IVJ1
Organism   Dyella sp. BiH032     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1031962..1043020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RKE25_RS04560 (RKE25_04560) - 1032015..1033310 (+) 1296 WP_311841077.1 citrate synthase -
  RKE25_RS04565 (RKE25_04565) - 1033389..1034060 (-) 672 WP_311841078.1 hypothetical protein -
  RKE25_RS04570 (RKE25_04570) - 1034162..1036684 (-) 2523 WP_311841079.1 penicillin-binding protein 1A -
  RKE25_RS04575 (RKE25_04575) pilM 1036962..1038020 (+) 1059 WP_311841080.1 pilus assembly protein PilM Machinery gene
  RKE25_RS04580 (RKE25_04580) - 1038020..1038799 (+) 780 WP_311841081.1 PilN domain-containing protein -
  RKE25_RS04585 (RKE25_04585) pilO 1038796..1039449 (+) 654 WP_311841082.1 type 4a pilus biogenesis protein PilO -
  RKE25_RS04590 (RKE25_04590) - 1039446..1039976 (+) 531 WP_311841083.1 pilus assembly protein PilP -
  RKE25_RS04595 (RKE25_04595) - 1040033..1042228 (+) 2196 WP_311841084.1 type IV pilus secretin PilQ -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38025.67 Da        Isoelectric Point: 4.5868

>NTDB_id=884202 RKE25_RS04575 WP_311841080.1 1036962..1038020(+) (pilM) [Dyella sp. BiH032]
MGLFTPKKPPLIGVDISSTAVKLLQLSQAGGRYRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAVARSGTKLKHAAAA
VAGSAVITRIIPMSSDLSEEDLEGQIQVEANQYIPYPIEEVSLDFEVLGPVRDNPEMNNVLLAASRTENVDMRVAALDLG
GLAAKVVDVEAFAMENAFSMVADQLNVGRDALVAVVDIGATMTTLSVLRNQRTIYSREQVFGGKQLTDEIMRRYGLSYEE
AGRAKRKGGLPESYETEALEPFKESLIQQISRLLQFFFAGSEYSKVDQVVLAGGCASIEGLCPMLEEQLGVSCVVANPLA
RMSLSPRVQAQQLAQDAPALMIAVGLAMRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=884202 RKE25_RS04575 WP_311841080.1 1036962..1038020(+) (pilM) [Dyella sp. BiH032]
GTGGGGCTCTTTACACCCAAGAAGCCGCCGCTGATTGGCGTCGACATCAGTTCGACCGCCGTCAAGCTGCTGCAGCTCAG
TCAGGCCGGTGGCCGCTACCGCGTTGAGCATTACGCGGTCGAGCCGCTGCCCCCAAACGCCGTGGTCGAAAAGAACATCG
TCGAAGTCGAGGCGGTGGGCGAAGCGATTCGGCGCGCCGTCGCGCGATCGGGCACGAAGCTCAAGCACGCCGCCGCAGCC
GTCGCCGGTTCGGCGGTGATCACGCGCATCATTCCAATGTCGTCCGATCTGTCCGAAGAAGATCTCGAAGGACAGATACA
GGTCGAGGCGAACCAGTACATCCCGTATCCGATCGAGGAAGTAAGCCTCGACTTCGAAGTGCTGGGGCCGGTGCGCGACA
ACCCTGAAATGAACAATGTGTTGCTTGCTGCGTCCCGCACCGAGAACGTCGACATGCGCGTCGCCGCGCTCGACCTCGGC
GGACTCGCCGCGAAGGTGGTCGACGTCGAGGCGTTCGCGATGGAGAACGCGTTCTCGATGGTCGCCGACCAGCTCAACGT
CGGCCGCGACGCGCTCGTCGCCGTGGTCGACATCGGCGCCACCATGACCACGCTCTCGGTGCTGCGCAACCAGCGCACCA
TCTATTCGCGCGAGCAGGTCTTCGGCGGCAAGCAGCTCACCGACGAGATCATGCGCCGCTATGGCCTTTCCTACGAGGAA
GCCGGGCGCGCGAAGCGCAAGGGCGGCTTGCCGGAGTCCTACGAGACCGAGGCGCTGGAGCCCTTCAAGGAATCGCTGAT
CCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTTCGCGGGCAGCGAGTACAGCAAGGTCGACCAGGTGGTGCTCGCCGGCG
GCTGCGCGTCGATCGAGGGCCTGTGCCCGATGCTCGAGGAACAGCTCGGCGTGTCCTGCGTGGTCGCCAATCCGTTGGCG
CGCATGTCGCTGTCGCCGCGCGTGCAGGCGCAGCAGCTGGCCCAGGACGCGCCTGCGCTGATGATCGCGGTCGGCCTCGC
CATGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

53.448

98.864

0.528
  comM Acinetobacter nosocomialis M2

53.161

98.864

0.526
  comM Acinetobacter baylyi ADP1

51.143

99.432

0.509
  pilM Legionella pneumophila strain ERS1305867

43.626

100

0.437