Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   RMV17_RS01885 Genome accession   NZ_CP134772
Coordinates   437478..438542 (+) Length   354 a.a.
NCBI ID   WP_311885147.1    Uniprot ID   -
Organism   Pseudomonas sp. VD-NE ins     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 432478..443542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RMV17_RS01875 - 433204..434472 (+) 1269 WP_064117089.1 malic enzyme-like NAD(P)-binding protein -
  RMV17_RS01880 - 434815..437259 (-) 2445 WP_409373124.1 penicillin-binding protein 1A -
  RMV17_RS01885 pilM 437478..438542 (+) 1065 WP_311885147.1 pilus assembly protein PilM Machinery gene
  RMV17_RS01890 - 438542..439108 (+) 567 WP_034152958.1 PilN domain-containing protein -
  RMV17_RS01895 pilO 439105..439728 (+) 624 WP_016983853.1 type 4a pilus biogenesis protein PilO -
  RMV17_RS01900 - 439725..440252 (+) 528 WP_034152956.1 pilus assembly protein PilP -
  RMV17_RS01905 pilQ 440266..442344 (+) 2079 WP_311885152.1 type IV pilus secretin PilQ Machinery gene
  RMV17_RS01910 aroK 442349..442867 (+) 519 WP_025110261.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38079.67 Da        Isoelectric Point: 4.8109

>NTDB_id=883464 RMV17_RS01885 WP_311885147.1 437478..438542(+) (pilM) [Pseudomonas sp. VD-NE ins]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPVNAVVEKNIAELEGVGQAVSRVLVKARTGLKSVAV
AVAGSAVITKIIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVSVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYISEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLNTPTQVANP
FANMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=883464 RMV17_RS01885 WP_311885147.1 437478..438542(+) (pilM) [Pseudomonas sp. VD-NE ins]
GTGCTGGGACTCTTCAATAAAAAGGCCAATACGTTACTGGGGATCGACATCAGTTCCACATCAGTGAAGCTGCTGGAACT
GAGCCGTCAGGGTGATCGCTACCGGGTCGAGGCGTACGCGGTAGAGCCATTGCCTGTCAACGCCGTGGTCGAAAAGAACA
TCGCCGAGCTTGAAGGTGTCGGTCAGGCCGTGAGCCGCGTGCTGGTCAAGGCGCGCACCGGGCTCAAGAGCGTGGCGGTG
GCAGTGGCCGGTTCCGCCGTGATCACCAAGATCATCGAGATGGACGCCGGGCTGTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAAGCCGACCAATACATTCCCTATCCGCTGGACGAAGTTGCCATCGATTTCGAAGTCCAGGGCGTGTCGCCGC
GCAACCCCGAGCGGGTCAGCGTGCTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTTCGCGAGGCGGCGCTGGCCCTC
GCCGGGCTGACCGCGCGGGTAGTCGATGTCGAAGCCTACGCCCTGGAGCGCTCGTTCGGCTTGCTGGCAACACAACTGGC
CGCCTCTCAGGAGCGCCTGACCGTCGCCGTGGTCGACATCGGCGCGACCATGACCACCCTCAGCGTCCTGCACAACGGCA
AGATCATCTATACCCGCGAACAACTGTTCGGCGGTCGACAACTGACCGAGGAAATCCAGCGCCGCTATGGCCTGACCCTC
GAGCAGGCCGGGCTGGCGAAAAAGCAGGGCGGCCTGCCCGATGATTACATCAGCGAGGTCTTGCAACCCTTTCGCGAGGC
GCTGGTGCAGCAGGTGTCGCGCTCCTTGCAGTTCTTCTTCGCCTCCGGGCAATACAACGCGGTCGATCACATTCTGCTGG
CCGGCGGCACGGCGTCGGTGCCCGGGCTGGACCGCTTGATCGAGCAACGCCTGAACACGCCAACGCAAGTGGCCAACCCC
TTTGCCAACATGGCCTTGAGCAGCAAGGTCAACGCTGGGGCACTGGCCAGCGATGCGCCGGCTCTGATGATCGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

47.175

100

0.472