Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   AACG45_RS04120 Genome accession   NZ_CP151038
Coordinates   884868..886106 (+) Length   412 a.a.
NCBI ID   WP_073514647.1    Uniprot ID   -
Organism   Escherichia coli strain 19KM2326N     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 879868..891106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACG45_RS04095 mrcA 880148..882700 (-) 2553 WP_000673804.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  AACG45_RS04100 hofM 882820..883599 (+) 780 WP_001297394.1 DNA utilization protein HofM -
  AACG45_RS04105 hofN 883599..884138 (+) 540 WP_001069315.1 DNA utilization protein HofN -
  AACG45_RS04110 hofO 884122..884562 (+) 441 WP_001055763.1 DNA utilization protein HofO -
  AACG45_RS04115 hofP 884552..884956 (+) 405 WP_001264141.1 DNA utilization protein HofP -
  AACG45_RS04120 comE 884868..886106 (+) 1239 WP_073514647.1 DNA uptake porin HofQ Machinery gene
  AACG45_RS04125 aroK 886507..887028 (+) 522 WP_000818618.1 shikimate kinase AroK -
  AACG45_RS04130 aroB 887085..888173 (+) 1089 WP_000439850.1 3-dehydroquinate synthase -
  AACG45_RS04135 damX 888265..889551 (+) 1287 WP_000343178.1 cell division protein DamX -
  AACG45_RS04140 dam 889658..890494 (+) 837 WP_000742143.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44773.33 Da        Isoelectric Point: 6.5122

>NTDB_id=882647 AACG45_RS04120 WP_073514647.1 884868..886106(+) (comE) [Escherichia coli strain 19KM2326N]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAKQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEDNILSVHSIAWQNNNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=882647 AACG45_RS04120 WP_073514647.1 884868..886106(+) (comE) [Escherichia coli strain 19KM2326N]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTAAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGGCAGGAAGACAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATAACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGCTTGCTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.385

100

0.396
  comE Haemophilus influenzae 86-028NP

37.156

100

0.393
  pilQ Vibrio campbellii strain DS40M4

37.767

100

0.386
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.288

100

0.374
  pilQ Vibrio cholerae strain A1552

37.288

100

0.374
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364