Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   AACG32_RS18350 Genome accession   NZ_CP151009
Coordinates   3857448..3858941 (+) Length   497 a.a.
NCBI ID   WP_000249331.1    Uniprot ID   -
Organism   Escherichia coli strain 19KM1776     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3852448..3863941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACG32_RS18330 pilD 3853096..3853905 (+) 810 WP_048967826.1 prepilin peptidase PppA Machinery gene
  AACG32_RS18335 gspS2 3853971..3854381 (+) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  AACG32_RS18340 gspC 3854399..3855358 (+) 960 WP_048967818.1 type II secretion system protein GspC -
  AACG32_RS18345 gspD 3855388..3857448 (+) 2061 WP_000498829.1 type II secretion system secretin GspD -
  AACG32_RS18350 pilB/pilB1 3857448..3858941 (+) 1494 WP_000249331.1 type II secretion system ATPase GspE Machinery gene
  AACG32_RS18355 gspF 3858941..3860164 (+) 1224 WP_001544934.1 type II secretion system inner membrane protein GspF -
  AACG32_RS18360 gspG 3860181..3860636 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  AACG32_RS18365 gspH 3860640..3861203 (+) 564 WP_001115147.1 type II secretion system minor pseudopilin GspH -
  AACG32_RS18370 gspI 3861200..3861571 (+) 372 WP_000820090.1 type II secretion system minor pseudopilin GspI -
  AACG32_RS18375 gspJ 3861568..3862173 (+) 606 WP_001544933.1 type II secretion system minor pseudopilin GspJ -
  AACG32_RS18380 gspK 3862170..3863147 (+) 978 WP_000633203.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55214.35 Da        Isoelectric Point: 6.0089

>NTDB_id=882277 AACG32_RS18350 WP_000249331.1 3857448..3858941(+) (pilB/pilB1) [Escherichia coli strain 19KM1776]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=882277 AACG32_RS18350 WP_000249331.1 3857448..3858941(+) (pilB/pilB1) [Escherichia coli strain 19KM1776]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGTTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACACTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTTTGGCAGCGTGACTCTTCC
GAGGCTCGCCAGCTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCCGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGCCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGCAAACTGGCCTCGCTACTGGTATCGCGTATCAAGGTGATGGCGCGGCTGGACATTGCCGAAAAGCGTGTGCC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATCGACGTGCGCGTCTCCACCATGCCTTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCACGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGTAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTTGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTTCAGGCGTCGCTCACTGGGCACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTTTGACAGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGACTGCCGCCAGCCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAGACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.765

85.513

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilF Thermus thermophilus HB27

45

80.483

0.362