Detailed information NTG0881841

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RKQ56_RS14385
• Genome accession: NZ_CP134583
• Coordinates: 2991805..2992923 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain XH1032
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2986805..2997923

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RKQ56_RS14375 (RKQ56_14400)	-	2989921..2990613 (-)	693	WP_032030422.1	YggS family pyridoxal phosphate-dependent enzyme	-
RKQ56_RS14380 (RKQ56_14405)	pilT	2990740..2991777 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
RKQ56_RS14385 (RKQ56_14410)	pilU	2991805..2992923 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RKQ56_RS14390 (RKQ56_14415)	fur	2992993..2993430 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
RKQ56_RS14395 (RKQ56_14420)	-	2993542..2993940 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
RKQ56_RS14400 (RKQ56_14425)	-	2993995..2994318 (-)	324	WP_005139957.1	RnfH family protein	-
RKQ56_RS14405 (RKQ56_14430)	-	2994318..2995385 (-)	1068	WP_002084046.1	hypothetical protein	-
RKQ56_RS14410 (RKQ56_14435)	-	2995538..2995993 (-)	456	WP_000782976.1	bacteriohemerythrin	-
RKQ56_RS14415 (RKQ56_14440)	-	2996246..2997094 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=881841 RKQ56_RS14385 WP_000347036.1 2991805..2992923(+) (pilU) [Acinetobacter baumannii strain XH1032]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=881841 RKQ56_RS14385 WP_000347036.1 2991805..2992923(+) (pilU) [Acinetobacter baumannii strain XH1032]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTGGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCGCTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGAAAGCAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392