Detailed information NTG0881195

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RKQ70_RS14775
• Genome accession: NZ_CP134552
• Coordinates: 3072571..3073689 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain XH1047
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3067571..3078689

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RKQ70_RS14765 (RKQ70_14780)	-	3070687..3071379 (-)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-
RKQ70_RS14770 (RKQ70_14785)	pilT	3071506..3072543 (+)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
RKQ70_RS14775 (RKQ70_14790)	pilU	3072571..3073689 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RKQ70_RS14780 (RKQ70_14795)	fur	3073759..3074196 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
RKQ70_RS14785 (RKQ70_14800)	-	3074308..3074706 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
RKQ70_RS14790 (RKQ70_14805)	-	3074761..3075084 (-)	324	WP_000442585.1	RnfH family protein	-
RKQ70_RS14795 (RKQ70_14810)	-	3075084..3076151 (-)	1068	WP_000092403.1	hypothetical protein	-
RKQ70_RS14800 (RKQ70_14815)	-	3076304..3076759 (-)	456	WP_000782976.1	bacteriohemerythrin	-
RKQ70_RS14805 (RKQ70_14820)	-	3077012..3077860 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=881195 RKQ70_RS14775 WP_000347036.1 3072571..3073689(+) (pilU) [Acinetobacter baumannii strain XH1047]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=881195 RKQ70_RS14775 WP_000347036.1 3072571..3073689(+) (pilU) [Acinetobacter baumannii strain XH1047]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392