Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   RJW53_RS00805 Genome accession   NZ_CP134488
Coordinates   125954..126388 (+) Length   144 a.a.
NCBI ID   WP_074389248.1    Uniprot ID   A0AAP6A8C9
Organism   Streptococcus suis strain NLS50     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 127257..137539 125954..126388 flank 869


Gene organization within MGE regions


Location: 125954..137539
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJW53_RS00805 (RJW53_00805) comGF/cglF 125954..126388 (+) 435 WP_074389248.1 competence type IV pilus minor pilin ComGF Machinery gene
  RJW53_RS00810 (RJW53_00810) comGG 126366..126776 (+) 411 WP_074392769.1 competence type IV pilus minor pilin ComGG -
  RJW53_RS00815 (RJW53_00815) - 127257..127682 (-) 426 WP_226319165.1 ArpU family phage packaging/lysis transcriptional regulator -
  RJW53_RS00820 (RJW53_00820) - 128006..128581 (-) 576 WP_074392204.1 hypothetical protein -
  RJW53_RS00825 (RJW53_00825) - 128602..128775 (-) 174 WP_171840772.1 hypothetical protein -
  RJW53_RS00830 (RJW53_00830) - 129089..130525 (-) 1437 WP_257116214.1 phage/plasmid primase, P4 family -
  RJW53_RS00835 (RJW53_00835) - 130588..131466 (-) 879 WP_074389575.1 primase alpha helix C-terminal domain-containing protein -
  RJW53_RS00840 (RJW53_00840) - 131601..131885 (-) 285 WP_044766582.1 hypothetical protein -
  RJW53_RS00845 (RJW53_00845) - 131875..132213 (-) 339 WP_074390405.1 hypothetical protein -
  RJW53_RS00850 (RJW53_00850) - 132430..132873 (-) 444 WP_074390406.1 hypothetical protein -
  RJW53_RS00855 (RJW53_00855) - 133345..133971 (-) 627 WP_074390407.1 Rha family transcriptional regulator -
  RJW53_RS00860 (RJW53_00860) - 133988..134188 (-) 201 WP_015647585.1 helix-turn-helix transcriptional regulator -
  RJW53_RS00865 (RJW53_00865) - 134374..134883 (+) 510 WP_370695883.1 helix-turn-helix domain-containing protein -
  RJW53_RS00870 (RJW53_00870) - 135344..136489 (+) 1146 WP_074390408.1 site-specific integrase -
  RJW53_RS00875 (RJW53_00875) comYH 136586..137539 (+) 954 WP_074389250.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16432.74 Da        Isoelectric Point: 6.9832

>NTDB_id=880306 RJW53_RS00805 WP_074389248.1 125954..126388(+) (comGF/cglF) [Streptococcus suis strain NLS50]
MLKSKIPAFTLLECLVALVILSGSLLVFEGLSKLLVQEAHYQGQTVQKEWLVFSSQLRSEWDQAELVKVENGKVYVNKGE
QALAFGKSRSDDFRKTNDRGQGYQPMLYQVDSAAVSQENQLVRIDFNFKNGEERTFIYAFKEKS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=880306 RJW53_RS00805 WP_074389248.1 125954..126388(+) (comGF/cglF) [Streptococcus suis strain NLS50]
TTGTTAAAAAGTAAAATTCCTGCCTTTACTCTCTTGGAATGCTTGGTAGCCTTAGTCATCCTATCAGGAAGTCTTCTGGT
CTTTGAAGGCTTGTCAAAACTGCTGGTCCAAGAAGCGCATTACCAAGGTCAGACCGTTCAAAAGGAATGGTTGGTCTTCT
CTAGTCAATTGCGGTCGGAGTGGGACCAGGCTGAATTGGTCAAGGTTGAAAATGGCAAGGTCTATGTCAACAAAGGCGAA
CAGGCTTTGGCCTTTGGCAAGTCACGGTCGGATGATTTTCGGAAAACAAATGACAGGGGACAGGGTTACCAGCCCATGCT
CTATCAGGTGGATAGCGCAGCCGTTTCCCAAGAAAACCAGCTGGTGCGCATCGACTTTAACTTTAAAAACGGAGAGGAGC
GGACCTTTATCTATGCTTTTAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(37-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

52.482

97.917

0.514
  comGF/cglF Streptococcus pneumoniae D39

52.482

97.917

0.514
  comGF/cglF Streptococcus pneumoniae R6

52.482

97.917

0.514
  comGF/cglF Streptococcus pneumoniae TIGR4

52.482

97.917

0.514
  comYF Streptococcus mutans UA140

50

100

0.5
  comYF Streptococcus mutans UA159

49.306

100

0.493
  comGF/cglF Streptococcus mitis NCTC 12261

52.985

93.056

0.493
  comGF/cglF Streptococcus mitis SK321

50

93.056

0.465
  comGF Lactococcus lactis subsp. cremoris KW2

46.099

97.917

0.451