Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   RKE57_RS16920 Genome accession   NZ_CP134450
Coordinates   3671480..3672538 (-) Length   352 a.a.
NCBI ID   WP_010484061.1    Uniprot ID   A0A0L8AA94
Organism   Stenotrophomonas geniculata strain BR23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3666480..3677538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RKE57_RS16900 (RKE57_16900) - 3667409..3669382 (-) 1974 WP_049437119.1 type IV pilus secretin PilQ family protein -
  RKE57_RS16905 (RKE57_16905) - 3669404..3669910 (-) 507 WP_010484064.1 pilus assembly protein PilP -
  RKE57_RS16910 (RKE57_16910) pilO 3669937..3670593 (-) 657 WP_049410131.1 type 4a pilus biogenesis protein PilO -
  RKE57_RS16915 (RKE57_16915) - 3670590..3671480 (-) 891 WP_057502237.1 PilN domain-containing protein -
  RKE57_RS16920 (RKE57_16920) pilM 3671480..3672538 (-) 1059 WP_010484061.1 pilus assembly protein PilM Machinery gene
  RKE57_RS16925 (RKE57_16925) - 3672758..3675181 (+) 2424 WP_311068698.1 penicillin-binding protein 1A -
  RKE57_RS16930 (RKE57_16930) - 3675542..3676186 (+) 645 WP_012511978.1 hypothetical protein -
  RKE57_RS16935 (RKE57_16935) - 3676363..3677106 (-) 744 WP_311068699.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37753.41 Da        Isoelectric Point: 4.4089

>NTDB_id=880015 RKE57_RS16920 WP_010484061.1 3671480..3672538(-) (pilM) [Stenotrophomonas geniculata strain BR23]
MGLIPKSQSPLVGVDISSTAVKLLQLSRSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAMNRSGSKAKLAAAA
VAGSAVITKVIPMPAELDENDMEAQIELEAVNYIPYPIEEVNLDFEVIGAIPNNPEMVQVLLAASRSENVELRQSALELG
GLQAKVMDVEAFAVENAYALVASELPVSIEGVVALVDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEIMRRYGLSYEE
AGLAKRQGGLPESYEMEVLEPFKEATVQQISRLLQFFYAGSEFNRVDHIVLAGGCAVLGGLPEMVEEQLGVPTVVANPLA
QMTLGPKVNAHALAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=880015 RKE57_RS16920 WP_010484061.1 3671480..3672538(-) (pilM) [Stenotrophomonas geniculata strain BR23]
GTGGGGCTCATCCCAAAAAGTCAGTCGCCGCTTGTAGGCGTCGACATCAGTTCGACTGCGGTAAAGCTTTTGCAGCTGTC
CCGCAGTGGCAACCGTTTTCGTGTGGAACATTACGCCGTGGAACCTCTTCCGCCGAACGCGGTGGTGGAGAAGAACATCG
TGGAAGTGGAGGCCGTGGGTGAGGCCATCCGCCGTGCGATGAACCGCTCGGGGAGCAAGGCCAAGCTGGCCGCTGCCGCC
GTCGCCGGTTCGGCGGTGATCACCAAGGTGATCCCGATGCCGGCCGAGCTCGACGAAAACGACATGGAAGCCCAGATCGA
GCTGGAAGCGGTCAACTACATTCCGTACCCGATCGAGGAAGTGAACCTGGACTTCGAGGTGATCGGGGCGATCCCGAACA
ACCCGGAGATGGTCCAGGTGCTGCTGGCCGCGTCGCGTTCGGAGAACGTGGAACTGCGCCAGTCGGCCCTGGAACTGGGG
GGCCTGCAGGCCAAGGTGATGGACGTGGAGGCCTTCGCGGTCGAGAACGCCTACGCCCTGGTCGCCAGCGAGCTGCCGGT
GTCCATCGAGGGCGTGGTCGCGCTGGTCGATATCGGCGCCACCATGACCACCCTCAACGTCCTGCGCGGCGGCCGCAGCC
TGTACAGCCGCGAACAGGTGTTCGGTGGCAAGCAGCTGACCGACGAGATCATGCGCCGTTACGGCCTGAGCTACGAGGAA
GCCGGCCTGGCCAAGCGCCAGGGTGGGCTGCCGGAAAGCTACGAGATGGAAGTGCTGGAGCCGTTCAAGGAAGCCACGGT
CCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTACGCAGGCAGCGAGTTCAACCGCGTTGACCACATCGTGCTGGCCGGCG
GCTGCGCCGTGCTCGGCGGCCTGCCGGAAATGGTCGAGGAACAGCTGGGCGTGCCGACCGTGGTCGCCAACCCGCTGGCG
CAGATGACCCTGGGGCCGAAGGTGAACGCACATGCGCTGGCCCAGGATGCCCCCGCGCTGATGATCGCCACCGGTCTGGC
GCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0L8AA94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.136

100

0.511
  comM Acinetobacter nosocomialis M2

51.136

100

0.511
  comM Acinetobacter baylyi ADP1

50

100

0.5
  pilM Legionella pneumophila strain ERS1305867

47.578

99.716

0.474