Detailed information NTG0878798

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RGQ13_RS04320
• Genome accession: NZ_CP134145
• Coordinates: 1021520..1022632 (+)
• Length: 370 a.a.
• NCBI ID: WP_348392331.1
• Uniprot ID: -
• Organism: Thalassotalea psychrophila strain SQ149
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1016520..1027632

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RGQ13_RS04290 (RGQ13_04285)	-	1016973..1017563 (-)	591	WP_348392325.1	XTP/dITP diphosphatase	-
RGQ13_RS04295 (RGQ13_04290)	-	1017675..1018118 (-)	444	WP_348392326.1	DUF4426 domain-containing protein	-
RGQ13_RS04300 (RGQ13_04295)	-	1018229..1018777 (-)	549	WP_348392327.1	YggT family protein	-
RGQ13_RS04305 (RGQ13_04300)	proC	1018862..1019677 (-)	816	WP_348392328.1	pyrroline-5-carboxylate reductase	-
RGQ13_RS04310 (RGQ13_04305)	-	1019745..1020428 (-)	684	WP_348392329.1	YggS family pyridoxal phosphate-dependent enzyme	-
RGQ13_RS04315 (RGQ13_04310)	pilT	1020466..1021509 (+)	1044	WP_348392330.1	type IV pilus twitching motility protein PilT	Machinery gene
RGQ13_RS04320 (RGQ13_04315)	pilU	1021520..1022632 (+)	1113	WP_348392331.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RGQ13_RS04325 (RGQ13_04320)	-	1022660..1023391 (-)	732	WP_348392332.1	SapC family protein	-
RGQ13_RS04330 (RGQ13_04325)	-	1023410..1024966 (-)	1557	WP_348392333.1	tryptophan halogenase family protein	-
RGQ13_RS04335 (RGQ13_04330)	nhaC	1024963..1026468 (-)	1506	WP_348392334.1	Na+/H+ antiporter NhaC	-

Sequence

Protein

• Length: 370 a.a.
• Molecular weight: 40970.02 Da
• Isoelectric Point: 5.1606

>NTDB_id=878798 RGQ13_RS04320 WP_348392331.1 1021520..1022632(+) (pilU) [Thalassotalea psychrophila strain SQ149]
MYLHQLLKAMVKQDASDLFVTVNLSASAKINGELQPVDEKILNFDDCLELVHSSMTEKQITEFTETKECNYAIAIEDIGR
FRISAFWQREMPGMVVRRIVTDIPDTEALGLPPVLKELVMSKRGLLLFVGGTGTGKSTSMASLIGYRNRNARGHILTIED
PVEFVHEHGKSMITQREVGMDTESFDAALKSSLRQAPDVILIGEIRSQDIMEHALSFAETGHLCIATLHANNANQAIDRI
MHLVPPEQHEKLQFDLALNLRGIVAQQLVPTRDGLGRKAAIEILLNSPYISELIKKGAVGDIKEVMAKSRELGMQTFDQA
LFDLYEAGEISYTDALHNADSPNDLRLMIKLRSNDQTGVGGLKGMTLDGD

Nucleotide

• Length: 1113 bp

>NTDB_id=878798 RGQ13_RS04320 WP_348392331.1 1021520..1022632(+) (pilU) [Thalassotalea psychrophila strain SQ149]
ATGTATTTACACCAATTACTAAAAGCCATGGTGAAGCAAGATGCTTCTGATCTATTTGTTACAGTTAACCTTTCAGCAAG
TGCCAAAATAAATGGCGAATTACAGCCTGTAGATGAAAAGATATTAAACTTTGATGATTGTTTAGAGCTGGTTCATAGCT
CAATGACTGAAAAGCAAATTACTGAATTTACCGAAACAAAAGAATGTAACTATGCTATTGCTATTGAAGACATTGGCCGT
TTTCGTATTTCTGCATTTTGGCAACGTGAAATGCCAGGTATGGTTGTACGTCGAATTGTTACAGATATCCCCGATACCGA
GGCACTTGGTTTACCACCCGTTTTAAAAGAACTGGTGATGTCTAAACGTGGCTTATTGTTGTTCGTTGGTGGTACAGGTA
CTGGTAAATCAACATCGATGGCATCATTGATCGGTTATCGTAACCGTAATGCTCGTGGTCATATATTGACCATTGAAGAT
CCGGTAGAGTTTGTCCACGAACACGGTAAATCGATGATCACTCAACGAGAAGTTGGCATGGATACCGAGTCTTTTGATGC
GGCACTTAAAAGCTCATTACGCCAAGCTCCAGATGTTATCTTAATTGGTGAAATTCGCTCACAAGATATTATGGAGCACG
CATTAAGCTTTGCTGAAACTGGTCACTTATGTATTGCTACCTTACATGCCAATAATGCCAACCAAGCAATTGACCGTATC
ATGCATTTAGTACCGCCAGAGCAACATGAAAAGCTGCAGTTCGATTTAGCTTTGAATCTACGTGGTATTGTTGCCCAACA
GCTAGTACCAACTCGTGATGGTTTAGGCCGAAAAGCAGCTATTGAAATTTTACTGAACTCACCGTACATTTCTGAGCTCA
TTAAAAAAGGTGCTGTTGGAGACATCAAAGAAGTTATGGCGAAATCGCGCGAACTAGGCATGCAAACCTTCGATCAGGCG
TTATTTGATCTATACGAAGCAGGGGAGATAAGTTACACCGATGCCCTGCATAATGCTGATTCACCAAATGATTTGCGCTT
AATGATCAAACTTCGTAGTAATGATCAAACTGGAGTTGGTGGCCTCAAAGGTATGACCTTAGATGGTGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	59.375	95.135	0.565
pilU	Pseudomonas stutzeri DSM 10701	58.924	95.405	0.562
pilU	Vibrio cholerae strain A1552	55.435	99.459	0.551
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.73	96.216	0.392