Detailed information    

experimental Experimentally validated

Overview

Name   uvrB   Type   Machinery gene
Locus tag   SP_RS06070 Genome accession   NC_003028
Coordinates   1168313..1170301 (-) Length   662 a.a.
NCBI ID   WP_000607027.1    Uniprot ID   Q54986
Organism   Streptococcus pneumoniae TIGR4     
Function   homologous recombination   
Homologous recombination

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision.


Genomic Context

Location: 1163313..1175301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP_RS06030 (SP_1230) coaB 1163618..1164307 (+) 690 WP_000699501.1 phosphopantothenate--cysteine ligase -
  SP_RS06035 (SP_1231) coaC 1164319..1164870 (+) 552 WP_001284128.1 phosphopantothenoylcysteine decarboxylase -
  SP_RS06040 (SP_1232) - 1164854..1165417 (+) 564 WP_000747408.1 ECF transporter S component -
  SP_RS06045 (SP_1233) - 1165699..1166226 (+) 528 WP_001827349.1 ECF transporter S component -
  SP_RS06050 (SP_1234) - 1166238..1166753 (+) 516 WP_000159169.1 transcription repressor NadR -
  SP_RS06055 (SP_1235) - 1166750..1167217 (-) 468 WP_000091534.1 8-oxo-dGTP diphosphatase -
  SP_RS06060 (SP_1236) - 1167286..1167774 (-) 489 WP_000216002.1 GNAT family N-acetyltransferase -
  SP_RS06065 (SP_1237) - 1167740..1168303 (-) 564 WP_000639829.1 GNAT family N-acetyltransferase -
  SP_RS06070 (SP_1238) uvrB 1168313..1170301 (-) 1989 WP_000607027.1 excinuclease ABC subunit UvrB Machinery gene
  SP_RS06075 (SP_1240) - 1170378..1171331 (-) 954 WP_000792117.1 CPBP family intramembrane glutamic endopeptidase -
  SP_RS06080 (SP_1241) - 1171501..1173666 (+) 2166 WP_000726272.1 ABC transporter substrate-binding protein/permease -
  SP_RS06085 (SP_1242) - 1173666..1174406 (+) 741 WP_001096313.1 amino acid ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 662 a.a.        Molecular weight: 75733.76 Da        Isoelectric Point: 4.5848

>NTDB_id=690 SP_RS06070 WP_000607027.1 1168313..1170301(-) (uvrB) [Streptococcus pneumoniae TIGR4]
MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMGATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYG
EFKEFFPENAVEYFVSYYDYYQPEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFRVEFFGDEIDRIREVEALTGQVL
GEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQLAVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDG
RSEGEPPYTLLDFFPDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVHQIVYVSAT
PGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKNERTFITTLTKKMAEDLTDYFKEMGIKVKYM
HSDIKTLERTEIIRDLRLGVFDVLVGINLLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADT
VTQSMQRAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQERKELVKKLEKQMQEAV
EVLDFELAAQIRDMMLEVKALD

Nucleotide


Download         Length: 1989 bp        

>NTDB_id=690 SP_RS06070 WP_000607027.1 1168313..1170301(-) (uvrB) [Streptococcus pneumoniae TIGR4]
ATGATCAATCACATTACAGATAATCAATTTAAACTAGTATCAAAATATCAACCATCAGGAGATCAACCCCAAGCTATCGA
GCAGTTGGTGGATAACATTGAGGGGGGAGAAAAAGCTCAGATTCTGATGGGGGCGACTGGAACAGGGAAGACCTATACTA
TGAGTCAGGTCATTTCTAAAGTCAATAAACCAACTCTGGTTATTGCCCACAATAAAACTCTGGCTGGTCAGCTCTATGGG
GAGTTTAAGGAATTTTTCCCTGAAAATGCAGTTGAGTATTTCGTATCCTACTATGATTATTACCAGCCAGAGGCCTATGT
CCCTTCTAGCGATACCTATATTGAGAAGGATAGTTCTGTCAATGACGAGATTGACAAGCTTCGCCACTCAGCTACCTCAG
CCCTTTTGGAGCGTAATGATGTTATTGTCGTGGCCTCAGTCTCTTGTATCTATGGTTTGGGTTCGCCCAAGGAATACGCT
GATAGTGTCGTTAGTCTCCGTCCTGGTCTAGAGATTTCTCGTGATAAACTCTTGAATGACTTGGTCGATATTCAGTTTGA
ACGTAATGATATTGATTTCCAACGCGGAAGATTTCGCGTTCGTGGGGATGTGGTAGAGATTTTCCCAGCTTCCCGAGATG
AACATGCCTTTCGAGTAGAATTTTTTGGAGACGAAATTGACCGTATTCGTGAAGTTGAGGCTCTGACAGGTCAGGTGTTG
GGAGAAGTGGATCATTTAGCGATTTTCCCAGCGACACACTTTGTGACCAATGACGACCACATGGAAGTTGCCATTGCAAA
GATTCAGGCCGAGTTGGAAGAACAATTAGCTGTCTTTGAAAAGGAAGGTAAACTGCTTGAAGCCCAGCGTTTGAAACAGC
GGACAGAGTATGATATCGAAATGTTGCGTGAGATGGGCTATACCAATGGGGTTGAAAATTATTCTCGCCACATGGATGGA
CGGAGCGAAGGAGAGCCTCCTTATACGCTTCTCGACTTCTTCCCAGATGATTTCTTGATTATGATTGACGAGAGTCATAT
GACCATAGGGCAAATCAAGGGCATGTACAATGGAGACCGTTCGCGTAAAGAAATGCTGGTTAATTATGGTTTCCGTTTGC
CGTCTGCTTTGGACAATCGTCCTCTCCGTCGGGAGGAGTTTGAGAGTCACGTTCATCAGATTGTTTACGTTTCAGCGACA
CCTGGTGACTATGAAAATGAACAGACCGAGACAGTGATTGAGCAAATCATTCGTCCAACGGGACTCTTGGATCCAGAGGT
GGAAGTCCGTCCGACTATGGGACAGATTGATGACCTCTTGGGTGAAATCAATGCCCGCGTTGAAAAAAATGAGCGTACCT
TTATCACAACTTTGACCAAGAAAATGGCAGAGGATTTGACCGACTACTTCAAGGAAATGGGTATCAAGGTCAAGTACATG
CACTCGGATATCAAGACCTTGGAACGGACGGAGATTATCCGTGACCTGCGCTTGGGTGTCTTTGATGTCTTGGTCGGAAT
TAACCTGCTCCGTGAAGGAATTGACGTTCCTGAAGTGAGCCTCGTAGCTATTCTCGATGCTGACAAGGAAGGTTTCCTTC
GCAACGAACGTGGACTCATCCAGACCATTGGACGTGCTGCACGTAATAGCGAAGGTCATGTTATCATGTATGCGGACACG
GTTACCCAGTCTATGCAACGTGCTATCGATGAAACTGCCCGCCGTCGCAAAATCCAGATGGCCTATAATGAAGAACATGG
TATCGTTCCACAAACCATCAAGAAAGAAATCCGTGACTTGATTGCTGTGACCAAGGCAGTTGCTAAGGAAGAAGACAAGG
AAGTCGATATCAATAGCCTCAACAAACAAGAGCGCAAAGAACTAGTCAAAAAGCTTGAGAAACAAATGCAAGAAGCAGTT
GAAGTGCTTGACTTTGAACTAGCAGCTCAGATTCGTGATATGATGCTGGAAGTCAAGGCCTTGGATTAG

Domains

Predicted by InterProScan.

Ultra-violet resistance protein B (UvrB)

(556-598)

UvrB/uvrC motif (UVR)

(626-660)

Helicase conserved C-terminal domain (Helicase_C)

(443-549)

Type III restriction enzyme, res subunit (ResIII)

(17-143)

UvrB interaction domain (UvrB_inter)

(164-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q54986

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  uvrB Streptococcus pneumoniae D39

99.849

100

0.998
  uvrB Streptococcus pneumoniae R6

99.849

100

0.998

References

[1] H Tettelin et al. (2001) Complete genome sequence of a virulent isolate of Streptococcus pneumoniae. Science (New York, N.Y.) 293(5529):498-506. [PMID: 11463916]