Detailed information NTG0000689

Experimentally validated

Overview

• Name: uvrB
• Type: Machinery gene
• Locus tag: SPD_RS05865
• Genome accession: NC_008533
• Coordinates: 1123999..1125987 (-)
• Length: 662 a.a.
• NCBI ID: WP_000607031.1
• Uniprot ID: Q8DPK7
• Organism: Streptococcus pneumoniae D39
• Function: homologous recombination Homologous recombination

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision.

Genomic Context

Location: 1118999..1130987

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPD_RS05825 (SPD_1088)	coaB	1119299..1119988 (+)	690	WP_000699498.1	phosphopantothenate--cysteine ligase	-
SPD_RS05830 (SPD_1089)	coaC	1120000..1120551 (+)	552	WP_001284128.1	phosphopantothenoylcysteine decarboxylase	-
SPD_RS05835 (SPD_1090)	-	1120535..1121098 (+)	564	WP_000747408.1	ECF transporter S component	-
SPD_RS05840 (SPD_1091)	-	1121380..1121907 (+)	528	WP_001097402.1	ECF transporter S component	-
SPD_RS05845 (SPD_1093)	-	1121919..1122434 (+)	516	WP_000159176.1	transcription repressor NadR	-
SPD_RS05850 (SPD_1092)	-	1122431..1122898 (-)	468	WP_000091534.1	8-oxo-dGTP diphosphatase	-
SPD_RS05855 (SPD_1094)	-	1122972..1123460 (-)	489	WP_000216018.1	GNAT family N-acetyltransferase	-
SPD_RS05860 (SPD_1095)	-	1123426..1123989 (-)	564	WP_000639832.1	GNAT family N-acetyltransferase	-
SPD_RS05865 (SPD_1096)	uvrB	1123999..1125987 (-)	1989	WP_000607031.1	excinuclease ABC subunit UvrB	Machinery gene
SPD_RS05870 (SPD_1097)	-	1126064..1127017 (-)	954	WP_000792121.1	CPBP family intramembrane glutamic endopeptidase	-
SPD_RS05875 (SPD_1098)	-	1127190..1129355 (+)	2166	WP_000726272.1	ABC transporter substrate-binding protein/permease	-
SPD_RS05880 (SPD_1099)	-	1129355..1130095 (+)	741	WP_001096316.1	amino acid ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 662 a.a.
• Molecular weight: 75751.80 Da
• Isoelectric Point: 4.5848

>NTDB_id=689 SPD_RS05865 WP_000607031.1 1123999..1125987(-) (uvrB) [Streptococcus pneumoniae D39]
MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMGATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYG
EFKEFFPENAVEYFVSYYDYYQPEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFRVEFFGDEIDRIREVEALTGQVL
GEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQLAVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDG
RSEGEPPYTLLDFFPDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVHQIVYVSAT
PGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKNERTFITTLTKKMAEDLTDYFKEMGIKVKYM
HSDIKTLERTEIIRDLRLGVFDVLVGINLLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADT
VTQSMQRAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQERKELVKKLEKQMQEAV
EVLDFELAAQIRDMMLEVKALD

Nucleotide

• Length: 1989 bp

>NTDB_id=689 SPD_RS05865 WP_000607031.1 1123999..1125987(-) (uvrB) [Streptococcus pneumoniae D39]
ATGATCAATCACATTACAGATAATCAATTTAAACTAGTATCAAAATATCAACCATCAGGAGATCAACCCCAAGCTATCGA
GCAGTTGGTGGATAACATTGAGGGGGGAGAAAAAGCTCAGATTCTGATGGGGGCGACTGGAACAGGGAAGACCTATACTA
TGAGTCAGGTCATTTCTAAAGTCAATAAACCAACTCTGGTTATTGCCCACAATAAAACTCTGGCTGGTCAGCTCTATGGG
GAGTTTAAGGAATTTTTCCCTGAAAATGCAGTTGAGTATTTCGTATCCTACTATGATTATTACCAGCCAGAGGCCTATGT
CCCTTCTAGCGATACCTATATTGAGAAGGATAGTTCTGTCAATGACGAGATTGACAAGCTTCGCCACTCAGCTACCTCAG
CCCTTTTGGAGCGTAATGATGTTATTGTCGTGGCCTCAGTCTCTTGTATCTATGGTTTGGGTTCGCCCAAGGAATACGCT
GATAGTGTCGTTAGTCTCCGTCCTGGTCTAGAGATTTCTCGTGATAAACTCTTGAATGACTTGGTCGATATTCAGTTTGA
ACGTAATGATATTGATTTCCAACGCGGAAGATTTCGCGTTCGTGGGGATGTGGTAGAGATTTTCCCAGCTTCCCGAGATG
AACATGCCTTTCGAGTAGAATTTTTTGGAGACGAAATTGACCGTATCCGTGAGGTTGAGGCTCTGACAGGTCAGGTATTG
GGAGAAGTGGATCATTTAGCGATTTTCCCAGCGACACACTTTGTGACCAATGACGACCACATGGAAGTTGCCATTGCAAA
GATTCAGGCCGAGTTGGAAGAACAATTAGCTGTCTTTGAAAAGGAAGGTAAACTGCTTGAAGCCCAGCGTTTGAAACAGC
GGACAGAGTATGATATCGAAATGCTGCGCGAGATGGGCTATACAAATGGGGTTGAAAATTATTCTCGCCACATGGATGGA
CGTAGTGAAGGTGAGCCTCCTTATACGCTTCTCGACTTTTTCCCAGATGATTTTTTGATTATGATTGACGAGAGTCATAT
GACTATGGGACAAATAAAAGGCATGTACAATGGAGACCGTTCGCGTAAGGAAATGCTAGTAAATTATGGTTTCCGTTTAC
CGTCTGCCTTGGACAATCGTCCTCTCCGTCGTGAGGAGTTTGAAAGCCATGTGCATCAGATTGTTTACGTATCAGCGACA
CCTGGTGACTATGAAAATGAACAGACTGAGACGGTGATTGAGCAAATCATTCGACCGACAGGGCTTTTGGATCCTGAGGT
GGAAGTCCGTCCGACTATGGGACAGATTGATGATCTCTTAGGTGAAATCAATGCCCGTGTTGAAAAGAATGAACGAACCT
TTATCACCACCTTGACCAAGAAAATGGCAGAGGACTTGACCGACTATTTCAAGGAAATGGGCATCAAGGTCAAGTACATG
CACTCGGATATCAAGACCTTGGAACGGACGGAGATTATCCGTGATCTCCGCTTGGGTGTCTTTGATGTCTTGGTTGGAAT
CAATCTCCTTCGTGAAGGGATTGACGTTCCTGAGGTTAGCTTGGTTGCCATTCTCGATGCTGACAAGGAAGGTTTCCTTC
GTAACGAGCGTGGACTCATCCAGACCATTGGGCGTGCCGCCCGTAACAGTGAAGGTCATGTCATCATGTATGCAGATACG
GTTACCCAGTCTATGCAACGTGCTATCGATGAAACTGCCCGCCGTCGGAAAATTCAGATGGCCTATAATGAAGAGCATGG
TATCGTACCACAGACAATCAAGAAAGAAATCCGTGACCTGATTGCCGTGACCAAGGCAGTTGCTAAGGAAGAGGACAAGG
AAGTCGACATCAATAGCCTCAACAAACAAGAGCGCAAAGAACTCGTCAAGAAACTAGAAAAACAAATGCAAGAAGCCGTC
GAAGTGCTTGACTTTGAACTGGCGGCTCAGATCCGTGATATGATGTTGGAAGTCAAGGCCTTGGATTAG

Domains

Predicted by InterProScan.

• Ultra-violet resistance protein B (UvrB) (556-598)

• UvrB/uvrC motif (UVR) (626-660)

• Type III restriction enzyme, res subunit (ResIII) (17-143)

• Helicase conserved C-terminal domain (Helicase_C) (443-549)

• UvrB interaction domain (UvrB_inter) (164-254)

3D structure

• AlphaFold DB: Q8DPK7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
uvrB	Streptococcus pneumoniae R6	100	100	1
uvrB	Streptococcus pneumoniae TIGR4	99.849	100	0.998

References

• [1]: Joel A Lanie et al. (2007) Genome sequence of Avery's virulent serotype 2 strain D39 of Streptococcus pneumoniae and comparison with that of unencapsulated laboratory strain R6. Journal of Bacteriology 189(1):38-51. [PMID: 17041037]