Detailed information    

experimental Experimentally validated

Overview

Name   uvrB   Type   Machinery gene
Locus tag   SPR_RS05580 Genome accession   NC_003098
Coordinates   1116467..1118455 (-) Length   662 a.a.
NCBI ID   WP_000607031.1    Uniprot ID   Q8DPK7
Organism   Streptococcus pneumoniae R6     
Function   homologous recombination   
Homologous recombination

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision.


Genomic Context

Location: 1111467..1123455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPR_RS05540 (spr1110) coaB 1111767..1112456 (+) 690 WP_000699498.1 phosphopantothenate--cysteine ligase -
  SPR_RS05545 (spr1111) coaC 1112468..1113019 (+) 552 WP_001284128.1 phosphopantothenoylcysteine decarboxylase -
  SPR_RS05550 (spr1112) - 1113003..1113566 (+) 564 WP_000747408.1 ECF transporter S component -
  SPR_RS05555 (spr1113) - 1113848..1114375 (+) 528 WP_001097402.1 ECF transporter S component -
  SPR_RS05560 (spr1114) - 1114387..1114902 (+) 516 WP_000159176.1 transcription repressor NadR -
  SPR_RS05565 (spr1115) - 1114899..1115366 (-) 468 WP_000091534.1 8-oxo-dGTP diphosphatase -
  SPR_RS05570 (spr1116) - 1115440..1115928 (-) 489 WP_000216018.1 GNAT family N-acetyltransferase -
  SPR_RS05575 (spr1117) - 1115894..1116457 (-) 564 WP_000639832.1 GNAT family N-acetyltransferase -
  SPR_RS05580 (spr1118) uvrB 1116467..1118455 (-) 1989 WP_000607031.1 excinuclease ABC subunit UvrB Machinery gene
  SPR_RS05585 (spr1119) - 1118532..1119485 (-) 954 WP_000792121.1 CPBP family intramembrane glutamic endopeptidase -
  SPR_RS05590 (spr1120) - 1119658..1121823 (+) 2166 WP_000726269.1 ABC transporter substrate-binding protein/permease -
  SPR_RS05595 (spr1121) - 1121823..1122563 (+) 741 WP_001096316.1 amino acid ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 662 a.a.        Molecular weight: 75751.80 Da        Isoelectric Point: 4.5848

>NTDB_id=691 SPR_RS05580 WP_000607031.1 1116467..1118455(-) (uvrB) [Streptococcus pneumoniae R6]
MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMGATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYG
EFKEFFPENAVEYFVSYYDYYQPEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFRVEFFGDEIDRIREVEALTGQVL
GEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQLAVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDG
RSEGEPPYTLLDFFPDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVHQIVYVSAT
PGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKNERTFITTLTKKMAEDLTDYFKEMGIKVKYM
HSDIKTLERTEIIRDLRLGVFDVLVGINLLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADT
VTQSMQRAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQERKELVKKLEKQMQEAV
EVLDFELAAQIRDMMLEVKALD

Nucleotide


Download         Length: 1989 bp        

>NTDB_id=691 SPR_RS05580 WP_000607031.1 1116467..1118455(-) (uvrB) [Streptococcus pneumoniae R6]
ATGATCAATCACATTACAGATAATCAATTTAAACTAGTATCAAAATATCAACCATCAGGAGATCAACCCCAAGCTATCGA
GCAGTTGGTGGATAACATTGAGGGGGGAGAAAAAGCTCAGATTCTGATGGGGGCGACTGGAACAGGGAAGACCTATACTA
TGAGTCAGGTCATTTCTAAAGTCAATAAACCAACTCTGGTTATTGCCCACAATAAAACTCTGGCTGGTCAGCTCTATGGG
GAGTTTAAGGAATTTTTCCCTGAAAATGCAGTTGAGTATTTCGTATCCTACTATGATTATTACCAGCCAGAGGCCTATGT
CCCTTCTAGCGATACCTATATTGAGAAGGATAGTTCTGTCAATGACGAGATTGACAAGCTTCGCCACTCAGCTACCTCAG
CCCTTTTGGAGCGTAATGATGTTATTGTCGTGGCCTCAGTCTCTTGTATCTATGGTTTGGGTTCGCCCAAGGAATACGCT
GATAGTGTCGTTAGTCTCCGTCCTGGTCTAGAGATTTCTCGTGATAAACTCTTGAATGACTTGGTCGATATTCAGTTTGA
ACGTAATGATATTGATTTCCAACGCGGAAGATTTCGCGTTCGTGGGGATGTGGTAGAGATTTTCCCAGCTTCCCGAGATG
AACATGCCTTTCGAGTAGAATTTTTTGGAGACGAAATTGACCGTATCCGTGAGGTTGAGGCTCTGACAGGTCAGGTATTG
GGAGAAGTGGATCATTTAGCGATTTTCCCAGCGACACACTTTGTGACCAATGACGACCACATGGAAGTTGCCATTGCAAA
GATTCAGGCCGAGTTGGAAGAACAATTAGCTGTCTTTGAAAAGGAAGGTAAACTGCTTGAAGCCCAGCGTTTGAAACAGC
GGACAGAGTATGATATCGAAATGCTGCGCGAGATGGGCTATACAAATGGGGTTGAAAATTATTCTCGCCACATGGATGGA
CGTAGTGAAGGTGAGCCTCCTTATACGCTTCTCGACTTTTTCCCAGATGATTTTTTGATTATGATTGACGAGAGTCATAT
GACTATGGGACAAATAAAAGGCATGTACAATGGAGACCGTTCGCGTAAGGAAATGCTAGTAAATTATGGTTTCCGTTTAC
CGTCTGCCTTGGACAATCGTCCTCTCCGTCGTGAGGAGTTTGAAAGCCATGTGCATCAGATTGTTTACGTATCAGCGACA
CCTGGTGACTATGAAAATGAACAGACTGAGACGGTGATTGAGCAAATCATTCGACCGACAGGGCTTTTGGATCCTGAGGT
GGAAGTCCGTCCGACTATGGGACAGATTGATGATCTCTTAGGTGAAATCAATGCCCGTGTTGAAAAGAATGAACGAACCT
TTATCACCACCTTGACCAAGAAAATGGCAGAGGACTTGACCGACTATTTCAAGGAAATGGGCATCAAGGTCAAGTACATG
CACTCGGATATCAAGACCTTGGAACGGACGGAGATTATCCGTGATCTCCGCTTGGGTGTCTTTGATGTCTTGGTTGGAAT
CAATCTCCTTCGTGAAGGGATTGACGTTCCTGAGGTTAGCTTGGTTGCCATTCTCGATGCTGACAAGGAAGGTTTCCTTC
GTAACGAGCGTGGACTCATCCAGACCATTGGGCGTGCCGCCCGTAACAGTGAAGGTCATGTCATCATGTATGCAGATACG
GTTACCCAGTCTATGCAACGTGCTATCGATGAAACTGCCCGCCGTCGGAAAATTCAGATGGCCTATAATGAAGAGCATGG
TATCGTACCACAGACAATCAAGAAAGAAATCCGTGACCTGATTGCCGTGACCAAGGCAGTTGCTAAGGAAGAGGACAAGG
AAGTCGACATCAATAGCCTCAACAAACAAGAGCGCAAAGAACTCGTCAAGAAACTAGAAAAACAAATGCAAGAAGCCGTC
GAAGTGCTTGACTTTGAACTGGCGGCTCAGATCCGTGATATGATGTTGGAAGTCAAGGCCTTGGATTAG

Domains

Predicted by InterProScan.

Ultra-violet resistance protein B (UvrB)

(556-598)

UvrB/uvrC motif (UVR)

(626-660)

Type III restriction enzyme, res subunit (ResIII)

(17-143)

Helicase conserved C-terminal domain (Helicase_C)

(443-549)

UvrB interaction domain (UvrB_inter)

(164-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8DPK7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  uvrB Streptococcus pneumoniae D39

100

100

1
  uvrB Streptococcus pneumoniae TIGR4

99.849

100

0.998

References

[1] J Hoskins et al. (2001) Genome of the bacterium Streptococcus pneumoniae strain R6. Journal of Bacteriology 183(19):5709-17. [PMID: 11544234]