Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RGQ13_RS04315 Genome accession   NZ_CP134145
Coordinates   1020466..1021509 (+) Length   347 a.a.
NCBI ID   WP_348392330.1    Uniprot ID   -
Organism   Thalassotalea psychrophila strain SQ149     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1015466..1026509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGQ13_RS04285 (RGQ13_04280) hemW 1015788..1016945 (-) 1158 WP_405054157.1 radical SAM family heme chaperone HemW -
  RGQ13_RS04290 (RGQ13_04285) - 1016973..1017563 (-) 591 WP_348392325.1 XTP/dITP diphosphatase -
  RGQ13_RS04295 (RGQ13_04290) - 1017675..1018118 (-) 444 WP_348392326.1 DUF4426 domain-containing protein -
  RGQ13_RS04300 (RGQ13_04295) - 1018229..1018777 (-) 549 WP_348392327.1 YggT family protein -
  RGQ13_RS04305 (RGQ13_04300) proC 1018862..1019677 (-) 816 WP_348392328.1 pyrroline-5-carboxylate reductase -
  RGQ13_RS04310 (RGQ13_04305) - 1019745..1020428 (-) 684 WP_348392329.1 YggS family pyridoxal phosphate-dependent enzyme -
  RGQ13_RS04315 (RGQ13_04310) pilT 1020466..1021509 (+) 1044 WP_348392330.1 type IV pilus twitching motility protein PilT Machinery gene
  RGQ13_RS04320 (RGQ13_04315) pilU 1021520..1022632 (+) 1113 WP_348392331.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RGQ13_RS04325 (RGQ13_04320) - 1022660..1023391 (-) 732 WP_348392332.1 SapC family protein -
  RGQ13_RS04330 (RGQ13_04325) - 1023410..1024966 (-) 1557 WP_348392333.1 tryptophan halogenase family protein -
  RGQ13_RS04335 (RGQ13_04330) nhaC 1024963..1026468 (-) 1506 WP_348392334.1 Na+/H+ antiporter NhaC -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38808.41 Da        Isoelectric Point: 5.8193

>NTDB_id=878797 RGQ13_RS04315 WP_348392330.1 1020466..1021509(+) (pilT) [Thalassotalea psychrophila strain SQ149]
MDITELLAFSVEHNASDLHLSTGMPPAIRVDGDVRKVNIPPLEDKDVNGLVYDIMTDRQRKEYEENLEVDFSFEVPNLAR
FRVNAFNQNRGPAAVFRTIPSKILSLDELGCPDIFRQISENPRGLVLVTGPTGSGKSTTLAAMVDYINETKRDHILTIED
PIEFVHDNKQCLINQREVHRDTLSFNNALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
VDVFPGEEKSMVRSMLSESLRAVISQTLIKKVGGGRVAAHEIMIGVPAIRNLIREDKIAQMYSAIQTGMSHGMQTMDQCL
QNLLSRGLITREDAMHKAADKNQFKVY

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=878797 RGQ13_RS04315 WP_348392330.1 1020466..1021509(+) (pilT) [Thalassotalea psychrophila strain SQ149]
ATGGATATTACCGAACTTTTAGCATTTAGTGTCGAACACAATGCTTCCGATTTACATTTATCTACTGGTATGCCACCTGC
CATTCGCGTTGATGGTGACGTACGTAAGGTTAACATCCCACCGCTAGAAGACAAAGACGTTAATGGTTTAGTGTATGACA
TTATGACCGATCGTCAACGTAAAGAGTACGAAGAAAATCTAGAAGTGGATTTCTCTTTTGAAGTACCTAACTTAGCTCGT
TTTCGTGTTAACGCATTTAACCAAAACCGTGGTCCTGCTGCAGTATTTCGTACCATCCCAAGTAAAATTTTGTCTTTAGA
TGAACTTGGCTGTCCTGATATTTTTAGACAAATCTCAGAAAACCCTCGTGGTTTAGTACTTGTTACCGGCCCAACAGGCT
CTGGTAAATCAACAACTCTAGCGGCTATGGTTGATTATATTAATGAAACTAAACGCGATCACATCTTAACTATTGAAGAT
CCTATCGAATTCGTTCATGACAACAAACAATGTTTAATTAACCAACGTGAAGTACATCGCGATACGTTAAGCTTTAACAA
TGCTCTGCGCTCAGCTCTGCGTGAAGATCCTGATGTAATACTTGTAGGTGAGATGCGTGATTTAGAAACAATCCGCTTAG
CAATGACCGCAGCAGAAACCGGTCATTTAGTATTTGGTACGCTCCATACTACCTCTGCACCAAAAACCATTGACCGTATT
GTTGATGTATTTCCAGGTGAAGAAAAAAGTATGGTGCGTTCTATGTTATCTGAATCACTTCGTGCGGTTATCTCGCAAAC
ATTAATCAAAAAAGTTGGTGGTGGTCGTGTTGCTGCCCATGAAATTATGATTGGTGTACCTGCCATTCGTAACTTAATTC
GTGAAGATAAAATTGCACAAATGTATTCAGCAATTCAAACCGGTATGTCACATGGCATGCAAACCATGGATCAATGTTTA
CAAAACTTATTAAGCCGTGGTCTTATTACCCGTGAAGATGCTATGCATAAAGCGGCGGATAAAAACCAGTTTAAGGTTTA
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.035

99.135

0.764
  pilT Pseudomonas stutzeri DSM 10701

76.453

99.135

0.758
  pilT Acinetobacter nosocomialis M2

76.163

99.135

0.755
  pilT Acinetobacter baumannii D1279779

75.872

99.135

0.752
  pilT Acinetobacter baumannii strain A118

75.872

99.135

0.752
  pilT Acinetobacter baylyi ADP1

75

99.135

0.744
  pilT Legionella pneumophila strain Lp02

72.674

99.135

0.72
  pilT Legionella pneumophila strain ERS1305867

72.674

99.135

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.623

97.118

0.686
  pilT Vibrio cholerae strain A1552

70.623

97.118

0.686
  pilT Neisseria meningitidis 8013

69.501

98.271

0.683
  pilT Neisseria gonorrhoeae MS11

69.208

98.271

0.68
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.623

99.424

0.533
  pilU Vibrio cholerae strain A1552

40.922

100

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.346

100

0.403
  pilU Acinetobacter baylyi ADP1

39.655

100

0.398