Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RHS39_RS13215 Genome accession   NZ_CP133900
Coordinates   2862224..2862688 (+) Length   154 a.a.
NCBI ID   WP_025789070.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain TW01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2857224..2867688
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHS39_RS13200 (RHS39_13200) pdhR 2859256..2860023 (-) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  RHS39_RS13205 (RHS39_13205) ampD 2860429..2860980 (-) 552 WP_005484832.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RHS39_RS13210 (RHS39_13210) nadC 2861073..2861960 (+) 888 WP_025547616.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RHS39_RS13215 (RHS39_13215) pilA 2862224..2862688 (+) 465 WP_025789070.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  RHS39_RS13220 (RHS39_13220) pilB 2862695..2864380 (+) 1686 WP_025789071.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RHS39_RS13225 (RHS39_13225) pilC 2864405..2865628 (+) 1224 WP_005479682.1 type II secretion system F family protein Machinery gene
  RHS39_RS13230 (RHS39_13230) pilD 2865693..2866562 (+) 870 WP_025622886.1 A24 family peptidase Machinery gene
  RHS39_RS13235 (RHS39_13235) coaE 2866563..2867177 (+) 615 WP_025789072.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16172.61 Da        Isoelectric Point: 8.1143

>NTDB_id=877429 RHS39_RS13215 WP_025789070.1 2862224..2862688(+) (pilA) [Vibrio parahaemolyticus strain TW01]
MRHSKQKKQQGFTLIELMIVVAIIGILAAFAVPAYQNYTKKATLAEFPKVAAAMKLAVELCAHENASDATTFKSNCLSGS
NGVPAKITNLNDISIEAVAGSSGVNVIAQAVVVKGPIAAKEQYIMASNYTGSGIEWTSSCKDAGGKAQTDYCPE

Nucleotide


Download         Length: 465 bp        

>NTDB_id=877429 RHS39_RS13215 WP_025789070.1 2862224..2862688(+) (pilA) [Vibrio parahaemolyticus strain TW01]
ATGAGACACAGTAAACAGAAAAAACAACAAGGTTTTACCTTGATTGAATTGATGATTGTAGTGGCTATTATCGGTATTTT
GGCTGCATTTGCCGTACCGGCATATCAAAACTATACAAAAAAAGCGACATTAGCGGAATTCCCCAAAGTAGCAGCAGCTA
TGAAATTAGCAGTTGAGTTGTGCGCACACGAAAATGCTTCGGATGCAACGACTTTTAAATCAAACTGTCTTTCCGGTAGT
AATGGGGTGCCTGCAAAGATTACTAACCTAAATGATATATCAATTGAAGCTGTCGCTGGGTCATCAGGTGTAAATGTTAT
TGCACAAGCAGTTGTAGTAAAAGGACCTATCGCAGCTAAAGAACAATACATAATGGCATCAAACTACACAGGAAGTGGCA
TTGAATGGACTAGCTCTTGTAAAGATGCAGGGGGTAAGGCTCAAACTGATTACTGCCCTGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

58.065

100

0.584
  pilA2 Legionella pneumophila strain ERS1305867

39.865

96.104

0.383
  pilA Haemophilus influenzae 86-028NP

41.429

90.909

0.377