Detailed information NTG0087679

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: MIN45_RS11155
• Genome accession: NZ_AP024718
• Coordinates: 2211867..2212928 (-)
• Length: 353 a.a.
• NCBI ID: WP_286292304.1
• Uniprot ID: -
• Organism: Methylomarinovum tepidoasis strain IN45
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2206867..2217928

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MIN45_RS11135 (MIN45_P2140)	-	2207970..2210030 (-)	2061	WP_286292295.1	type IV pilus secretin PilQ	-
MIN45_RS11140 (MIN45_P2141)	-	2210146..2210670 (-)	525	WP_286292298.1	pilus assembly protein PilP	-
MIN45_RS11145 (MIN45_P2142)	-	2210670..2211296 (-)	627	WP_286292300.1	type 4a pilus biogenesis protein PilO	-
MIN45_RS11150 (MIN45_P2143)	-	2211293..2211865 (-)	573	WP_286292302.1	PilN domain-containing protein	-
MIN45_RS11155 (MIN45_P2144)	pilM	2211867..2212928 (-)	1062	WP_286292304.1	pilus assembly protein PilM	Machinery gene
MIN45_RS11160 (MIN45_P2145)	-	2213087..2215387 (+)	2301	WP_286292305.1	penicillin-binding protein 1A	-
MIN45_RS11165 (MIN45_P2146)	-	2215390..2215971 (+)	582	WP_286292307.1	hypothetical protein	-
MIN45_RS11170 (MIN45_P2147)	-	2215968..2217281 (-)	1314	WP_286292308.1	citrate synthase	-
MIN45_RS11175 (MIN45_P2148)	rpmE	2217287..2217490 (-)	204	WP_286292311.1	50S ribosomal protein L31	-

Sequence

Protein

• Length: 353 a.a.
• Molecular weight: 38158.73 Da
• Isoelectric Point: 5.2867

>NTDB_id=87679 MIN45_RS11155 WP_286292304.1 2211867..2212928(-) (pilM) [Methylomarinovum tepidoasis strain IN45]
MMLFRRKQPPVLGIDISAAAVKLIELGRNNGRYRVESYGVEPLPPNAVVDKNIADVEAIGRAIKAAVKKSGTRLKHAAVA
VSGSAVITKTITLPAEYSEEDLEDQIQLEAEQYIPYALDEVSLDFEVIGPNAKNPKMNDVLLAASRRENVDNRVAALELA
GLKAAIVDVEAYALENAFTLIETQLAQHRKGGAIAIADVGATTTTFNVIHEGRIIYTREQGFGGRQLTEEIQRRYGLSYE
EAGLAKKHGGLPDSYMCEVLEPFKNAMAQQIGRSLQFFLSSSTHQDVDVLVLVGGCASIQDVDRLVAQKLGIPAVVANPF
VNMGLAGRVDAQALRQDAPAMMIACGLALRSFD

Nucleotide

• Length: 1062 bp

>NTDB_id=87679 MIN45_RS11155 WP_286292304.1 2211867..2212928(-) (pilM) [Methylomarinovum tepidoasis strain IN45]
GTGATGTTATTCAGACGCAAGCAACCGCCGGTTCTCGGCATCGACATCAGTGCGGCCGCGGTCAAATTGATCGAACTGGG
GCGCAACAACGGGCGTTACCGGGTGGAGAGTTATGGCGTCGAACCCCTGCCGCCCAACGCCGTGGTCGACAAGAACATCG
CCGATGTCGAGGCCATCGGCCGGGCGATCAAGGCGGCGGTGAAAAAGTCTGGAACGCGGCTGAAACATGCCGCCGTGGCA
GTTTCCGGTTCTGCGGTGATCACCAAGACGATCACCCTGCCCGCCGAGTATTCCGAAGAGGATCTGGAAGATCAGATTCA
GCTGGAGGCGGAGCAGTACATTCCCTATGCCCTCGACGAAGTCAGCCTCGATTTCGAGGTGATCGGGCCCAATGCCAAGA
ATCCGAAGATGAACGATGTTCTGCTGGCCGCGTCGCGTCGGGAGAACGTCGACAACCGGGTCGCGGCGTTAGAGCTTGCG
GGCCTGAAGGCGGCCATCGTCGATGTGGAAGCCTATGCACTTGAGAATGCTTTTACGTTGATAGAGACCCAGCTGGCGCA
GCATCGCAAAGGCGGGGCGATCGCGATCGCGGATGTGGGGGCGACGACGACCACCTTCAACGTCATTCACGAGGGGCGGA
TCATTTATACCCGCGAACAGGGTTTCGGCGGCCGTCAGCTGACCGAGGAGATTCAGCGCCGTTACGGCCTGTCCTACGAA
GAGGCAGGGCTTGCCAAGAAGCACGGTGGTCTGCCGGACAGCTACATGTGCGAGGTGCTCGAGCCTTTCAAAAATGCAAT
GGCGCAACAGATCGGCCGTTCGTTGCAGTTCTTCCTGTCCTCCAGCACCCACCAGGATGTGGATGTGCTGGTGCTGGTCG
GAGGATGCGCCTCCATCCAGGATGTCGACCGGCTGGTGGCGCAAAAGTTGGGGATTCCGGCCGTCGTAGCGAATCCGTTC
GTGAACATGGGCCTGGCCGGTCGGGTGGATGCCCAGGCGCTGCGTCAGGACGCTCCGGCGATGATGATCGCCTGTGGTTT
GGCTTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-352)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	50.708	100	0.507
comM	Acinetobacter nosocomialis M2	50.425	100	0.504
comM	Acinetobacter baylyi ADP1	48.159	100	0.482
pilM	Legionella pneumophila strain ERS1305867	40.793	100	0.408