Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RHM55_RS02685 Genome accession   NZ_CP133784
Coordinates   585765..586943 (-) Length   392 a.a.
NCBI ID   WP_322179393.1    Uniprot ID   -
Organism   Pseudomonas sp. MH9.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 580765..591943
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM55_RS02670 (RHM55_02670) - 581612..583003 (+) 1392 WP_322179390.1 GntP family permease -
  RHM55_RS02675 (RHM55_02675) - 583014..583784 (+) 771 WP_322179391.1 3-hydroxybutyrate dehydrogenase -
  RHM55_RS02680 (RHM55_02680) - 583797..585752 (+) 1956 WP_322179392.1 acetoacetate--CoA ligase -
  RHM55_RS02685 (RHM55_02685) pilU 585765..586943 (-) 1179 WP_322179393.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RHM55_RS02690 (RHM55_02690) - 587151..587426 (+) 276 WP_322179394.1 peptidylprolyl isomerase -
  RHM55_RS02695 (RHM55_02695) lpxO 587793..588692 (-) 900 WP_322179395.1 lipid A hydroxylase LpxO -
  RHM55_RS02700 (RHM55_02700) - 589243..589602 (+) 360 Protein_540 site-specific integrase -
  RHM55_RS02705 (RHM55_02705) - 589623..591212 (+) 1590 WP_322179396.1 ABC-F family ATPase -

Sequence

Protein


Download         Length: 392 a.a.        Molecular weight: 43250.40 Da        Isoelectric Point: 6.1434

>NTDB_id=876766 RHM55_RS02685 WP_322179393.1 585765..586943(-) (pilU) [Pseudomonas sp. MH9.2]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLRPLSTEALKGGEVATVAASIMDSEQKLEFERELEMNLAVSLAGIGR
FRINIFKQRNEVSIVARNIKLDIPRFEDLHLPPILLDVVMEKHGLVLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRQQLLHDLGNNLKAFVSQRLVKTLDGKRRAAVEVMLGTPTIRDLIQRNELGELKGIMEKSVNLGMQTFDSAL
FDLVIEGVISEEEALTNADSMNNVRLRLKLHRENGPATQITSAPAAPEPAAEVDVANWGLVDERDDGGPRRG

Nucleotide


Download         Length: 1179 bp        

>NTDB_id=876766 RHM55_RS02685 WP_322179393.1 585765..586943(-) (pilU) [Pseudomonas sp. MH9.2]
ATGGATTTCCCGGCGCTGTTGAAAATTCTGGCCAGCCAGGACGGATCGGACCTCTACCTGTCGACCGGCGCACCTCCCTG
CGCCAAATTCAATGGCGTGCTCAGGCCCCTGAGCACCGAAGCCCTGAAGGGCGGAGAGGTAGCGACAGTCGCCGCCAGCA
TCATGGACTCGGAGCAAAAGCTTGAGTTCGAGCGTGAGCTGGAAATGAACCTGGCGGTGTCGCTGGCCGGCATCGGTCGT
TTTCGGATCAACATTTTCAAGCAACGCAACGAAGTCTCGATCGTGGCGCGCAACATCAAGCTGGACATTCCTCGCTTCGA
AGACTTGCACCTGCCGCCGATCCTCCTCGATGTCGTGATGGAAAAACACGGCCTGGTCCTGTTCGTCGGTGCCACGGGTT
CGGGTAAGTCCACGTCGTTGGCGGCCTTGATCGACTACCGTAATCGCAATAGCAGCGGCCATATCATCACCATCGAAGAC
CCGGTGGAATATATCCATCGGCACAAAAAATCGATCATCAATCAACGCGAAGTGGGTGTGGACACCCGGAGCTTTCACGC
CGCCTTGAAGAACACTTTGCGCCAAGCGCCGGATGTGATTCTGATCGGTGAAATTCGTGACCGGGAGACCATGGAGCATG
CCCTGGCCTTTGCCGATACCGGTCACTTGGCGATTTCGACTTTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAGCGGCGCCAGCAGCTGTTGCATGATTTGGGTAACAACCTCAAAGCATTCGTTTCTCAGCG
GCTGGTCAAGACCCTGGATGGCAAACGACGGGCCGCTGTCGAAGTGATGCTGGGCACGCCGACCATTCGCGACCTCATTC
AACGCAATGAGCTGGGCGAGCTCAAAGGCATCATGGAGAAGTCGGTCAACCTCGGCATGCAAACGTTCGACAGCGCGTTG
TTCGATCTGGTGATTGAGGGCGTCATCAGTGAAGAGGAAGCCCTGACGAATGCCGACTCCATGAATAACGTGCGTCTGCG
CCTCAAACTGCACCGCGAAAATGGCCCTGCGACACAAATAACATCAGCCCCGGCGGCGCCCGAACCGGCCGCCGAAGTGG
ATGTTGCAAACTGGGGGCTGGTGGATGAAAGGGACGACGGTGGCCCGCGTCGCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.215

90.306

0.508
  pilU Acinetobacter baylyi ADP1

53.76

91.582

0.492
  pilU Vibrio cholerae strain A1552

50.992

90.051

0.459
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.295

94.133

0.37