Detailed information NTG0087647

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: MCIT9_RS10900
• Genome accession: NZ_AP024714
• Coordinates: 2136752..2137813 (-)
• Length: 353 a.a.
• NCBI ID: WP_317704913.1
• Uniprot ID: -
• Organism: Methylomarinovum caldicuralii strain IT-9
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2131752..2142813

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MCIT9_RS10880 (MIT9_P2097)	-	2132837..2134933 (-)	2097	WP_317704909.1	type IV pilus secretin PilQ	-
MCIT9_RS10885 (MIT9_P2098)	-	2135028..2135528 (-)	501	WP_317704910.1	pilus assembly protein PilP	-
MCIT9_RS10890 (MIT9_P2099)	-	2135552..2136181 (-)	630	WP_317704911.1	type 4a pilus biogenesis protein PilO	-
MCIT9_RS10895 (MIT9_P2100)	-	2136178..2136750 (-)	573	WP_317704912.1	PilN domain-containing protein	-
MCIT9_RS10900 (MIT9_P2101)	pilM	2136752..2137813 (-)	1062	WP_317704913.1	pilus assembly protein PilM	Machinery gene
MCIT9_RS10905 (MIT9_P2102)	-	2137972..2140272 (+)	2301	WP_317704914.1	penicillin-binding protein 1A	-
MCIT9_RS10910 (MIT9_P2103)	-	2140275..2140859 (+)	585	WP_317704915.1	hypothetical protein	-
MCIT9_RS10915 (MIT9_P2104)	-	2140856..2142169 (-)	1314	WP_317704916.1	citrate synthase	-
MCIT9_RS10920 (MIT9_P2105)	rpmE	2142175..2142378 (-)	204	WP_317704917.1	50S ribosomal protein L31	-

Sequence

Protein

• Length: 353 a.a.
• Molecular weight: 38190.71 Da
• Isoelectric Point: 5.1369

>NTDB_id=87647 MCIT9_RS10900 WP_317704913.1 2136752..2137813(-) (pilM) [Methylomarinovum caldicuralii strain IT-9]
MMLFRRKQPPVLGIDISAAAVKLIELGRSNGRYRVESYGVEPLPPNAVVDRNIADVEAIGRAIKAAVKKSGTRLKHAAVA
VSGSAVITKTITLPAEYSEQDLEDQIQLEAEQYIPYSLDEVSLDFEVLGPNAKNPEMNDILLAASRRENVDNQVAALELA
GLKAAIVDVEACALENAFTLIETQLAQYRKGGAIALADVGATTTTFNVIHEGRIIYTREQGFGGRQLTEEIQRRYGLSYE
EAGLAKKHGGLPDSYVREVLEPFKNAMAQQIGRSLQFFLSSSAHRDVDVLVLVGGCASIQDVDRLVAQKLGIPAVVANPF
VNMALASRVDAQALRQDAPAMMIACGLALRSFD

Nucleotide

• Length: 1062 bp

>NTDB_id=87647 MCIT9_RS10900 WP_317704913.1 2136752..2137813(-) (pilM) [Methylomarinovum caldicuralii strain IT-9]
GTGATGTTATTCAGACGCAAGCAACCGCCGGTTCTCGGCATCGACATCAGTGCGGCCGCGGTCAAGCTGATCGAGTTGGG
GCGCAGCAACGGGCGTTACCGGGTGGAGAGTTATGGGGTCGAGCCCCTGCCGCCCAACGCCGTGGTCGACAGGAATATCG
CCGATGTCGAGGCCATCGGCCGGGCGATCAAGGCGGCGGTGAAGAAGTCGGGAACACGGCTGAAGCACGCTGCCGTGGCG
GTTTCCGGTTCCGCGGTGATCACCAAGACGATCACCCTGCCCGCCGAGTATTCCGAGCAGGATCTGGAGGATCAGATTCA
GCTGGAAGCGGAGCAGTACATCCCTTATTCCCTCGACGAGGTCAGCCTCGATTTCGAGGTGCTCGGACCCAACGCCAAGA
ATCCGGAGATGAACGACATTCTGCTGGCCGCGTCGCGGCGGGAGAACGTCGACAACCAAGTCGCGGCGTTAGAGCTGGCG
GGCCTGAAGGCGGCCATCGTCGATGTGGAAGCCTGTGCGCTCGAGAATGCCTTTACGCTGATAGAGACCCAGCTGGCGCA
GTATCGCAAAGGCGGGGCGATCGCGCTCGCGGATGTGGGGGCAACGACGACCACCTTCAACGTCATTCACGAGGGGCGGA
TCATTTATACCCGCGAACAGGGCTTCGGCGGCCGCCAGCTGACCGAGGAGATCCAGCGCCGCTACGGGCTTTCCTACGAG
GAGGCGGGGCTGGCCAAGAAGCACGGGGGGCTGCCGGACAGCTACGTGCGCGAGGTCCTCGAGCCCTTCAAGAATGCGAT
GGCGCAGCAGATCGGCCGTTCGCTGCAGTTCTTCCTGTCCTCCAGCGCCCACCGGGATGTGGACGTGCTGGTGCTGGTCG
GAGGGTGTGCCTCCATTCAGGATGTTGACCGGCTGGTGGCGCAGAAGCTGGGGATTCCGGCCGTCGTGGCGAATCCGTTC
GTGAACATGGCCCTGGCCAGCCGGGTGGATGCCCAGGCGCTGCGTCAGGACGCTCCGGCGATGATGATCGCCTGTGGTTT
GGCTTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-352)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	49.292	100	0.493
comM	Acinetobacter nosocomialis M2	49.008	100	0.49
comM	Acinetobacter baylyi ADP1	47.009	99.433	0.467
pilM	Legionella pneumophila strain ERS1305867	40.227	100	0.402