Detailed information NTG0876326

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RDJ20_RS02240
• Genome accession: NZ_CP133755
• Coordinates: 488823..489971 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain BJ86
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 483823..494971

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RDJ20_RS02215 (RDJ20_02215)	-	484974..485270 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
RDJ20_RS02220 (RDJ20_02220)	-	485267..485860 (-)	594	WP_003084544.1	YggT family protein	-
RDJ20_RS02225 (RDJ20_02225)	proC	485871..486692 (-)	822	WP_033977342.1	pyrroline-5-carboxylate reductase	-
RDJ20_RS02230 (RDJ20_02230)	-	486704..487396 (-)	693	WP_033972290.1	YggS family pyridoxal phosphate-dependent enzyme	-
RDJ20_RS02235 (RDJ20_02235)	pilT	487611..488645 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
RDJ20_RS02240 (RDJ20_02240)	pilU	488823..489971 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
RDJ20_RS02245 (RDJ20_02245)	czcD	489979..490878 (-)	900	WP_033977343.1	CDF family zinc transporter CzcD	-
RDJ20_RS02250 (RDJ20_02250)	-	491032..491436 (+)	405	WP_003084560.1	NINE protein	-
RDJ20_RS02255 (RDJ20_02255)	-	491679..493052 (+)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
RDJ20_RS02260 (RDJ20_02260)	-	493049..494233 (+)	1185	WP_033972291.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=876326 RDJ20_RS02240 WP_003100959.1 488823..489971(+) (pilU) [Pseudomonas aeruginosa strain BJ86]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=876326 RDJ20_RS02240 WP_003100959.1 488823..489971(+) (pilU) [Pseudomonas aeruginosa strain BJ86]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGTTGTCGCCGGAACAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGACTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTTTTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGATTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTACACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCCGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382