Detailed information NTG0876315

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RG643_RS25515
• Genome accession: NZ_CP133753
• Coordinates: 5459273..5460421 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain ZYPA187
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5454273..5465421

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RG643_RS25490 (RG643_25485)	-	5455424..5455720 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
RG643_RS25495 (RG643_25490)	-	5455717..5456310 (-)	594	WP_003084544.1	YggT family protein	-
RG643_RS25500 (RG643_25495)	proC	5456321..5457142 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
RG643_RS25505 (RG643_25500)	-	5457154..5457846 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
RG643_RS25510 (RG643_25505)	pilT	5458061..5459095 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
RG643_RS25515 (RG643_25510)	pilU	5459273..5460421 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
RG643_RS25520 (RG643_25515)	czcD	5460429..5461328 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
RG643_RS25525 (RG643_25520)	-	5461483..5461887 (+)	405	WP_003084560.1	NINE protein	-
RG643_RS25530 (RG643_25525)	-	5462130..5463503 (+)	1374	WP_015502220.1	pyridoxal-phosphate dependent enzyme	-
RG643_RS25535 (RG643_25530)	-	5463500..5464684 (+)	1185	WP_044061315.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=876315 RG643_RS25515 WP_003100959.1 5459273..5460421(+) (pilU) [Pseudomonas aeruginosa strain ZYPA187]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=876315 RG643_RS25515 WP_003100959.1 5459273..5460421(+) (pilU) [Pseudomonas aeruginosa strain ZYPA187]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATTACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGATTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382