Detailed information NTG0876262

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RG644_RS02400
• Genome accession: NZ_CP133751
• Coordinates: 499114..500262 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain ZYPA1927
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 494114..505262

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RG644_RS02380 (RG644_02380)	-	494851..496035 (-)	1185	WP_003100953.1	cystathionine gamma-synthase	-
RG644_RS02385 (RG644_02385)	-	496032..497405 (-)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
RG644_RS02390 (RG644_02390)	-	497648..498052 (-)	405	WP_003084560.1	NINE protein	-
RG644_RS02395 (RG644_02395)	czcD	498207..499106 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
RG644_RS02400 (RG644_02400)	pilU	499114..500262 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
RG644_RS02405 (RG644_02405)	pilT	500440..501474 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
RG644_RS02410 (RG644_02410)	-	501689..502381 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
RG644_RS02415 (RG644_02415)	proC	502393..503214 (+)	822	WP_031757004.1	pyrroline-5-carboxylate reductase	-
RG644_RS02420 (RG644_02420)	-	503225..503818 (+)	594	WP_003084544.1	YggT family protein	-
RG644_RS02425 (RG644_02425)	-	503815..504111 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=876262 RG644_RS02400 WP_003100959.1 499114..500262(-) (pilU) [Pseudomonas aeruginosa strain ZYPA1927]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=876262 RG644_RS02400 WP_003100959.1 499114..500262(-) (pilU) [Pseudomonas aeruginosa strain ZYPA1927]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382