Detailed information NTG0875974

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RGU80_RS14690
• Genome accession: NZ_CP133706
• Coordinates: 3020352..3021470 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain CRAb2
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3015352..3026470

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RGU80_RS14680 (RGU80_14710)	-	3018476..3019168 (-)	693	WP_001108520.1	YggS family pyridoxal phosphate-dependent enzyme	-
RGU80_RS14685 (RGU80_14715)	pilT	3019295..3020332 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
RGU80_RS14690 (RGU80_14720)	pilU	3020352..3021470 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RGU80_RS14695 (RGU80_14725)	fur	3021540..3021977 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
RGU80_RS14700 (RGU80_14730)	bamE	3022089..3022487 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
RGU80_RS14705 (RGU80_14735)	-	3022542..3022865 (-)	324	WP_000442589.1	RnfH family protein	-
RGU80_RS14710 (RGU80_14740)	-	3022865..3023932 (-)	1068	WP_029777448.1	membrane protein	-
RGU80_RS14715 (RGU80_14745)	-	3024085..3024540 (-)	456	WP_000782976.1	bacteriohemerythrin	-
RGU80_RS14720 (RGU80_14750)	-	3024793..3025641 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=875974 RGU80_RS14690 WP_000347036.1 3020352..3021470(+) (pilU) [Acinetobacter baumannii strain CRAb2]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=875974 RGU80_RS14690 WP_000347036.1 3020352..3021470(+) (pilU) [Acinetobacter baumannii strain CRAb2]
ATGGATTTTAATGACCTGCTCAACCTCATGGTTGAAAAAAAATCATCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTAAGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAGAGTGACCGTCATAGTCAGCTTTACATGGATTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392