Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   RFN66_RS14905 Genome accession   NZ_CP133705
Coordinates   2891060..2891689 (-) Length   209 a.a.
NCBI ID   WP_096748238.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain CP47     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2886060..2896689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN66_RS14885 (RFN66_14885) holA 2886619..2887644 (-) 1026 WP_188016690.1 DNA polymerase III subunit delta -
  RFN66_RS14890 (RFN66_14890) - 2887874..2888005 (+) 132 WP_003183686.1 YqzM family protein -
  RFN66_RS14895 (RFN66_14895) - 2888030..2890417 (-) 2388 WP_205802133.1 DNA internalization-related competence protein ComEC/Rec2 -
  RFN66_RS14900 (RFN66_14900) - 2890422..2890991 (-) 570 WP_020452273.1 ComE operon protein 2 -
  RFN66_RS14905 (RFN66_14905) comEA 2891060..2891689 (-) 630 WP_096748238.1 helix-hairpin-helix domain-containing protein Machinery gene
  RFN66_RS14910 (RFN66_14910) comER 2891772..2892593 (+) 822 WP_229128976.1 late competence protein ComER -
  RFN66_RS14915 (RFN66_14915) - 2892664..2893419 (-) 756 WP_023855255.1 class I SAM-dependent methyltransferase -
  RFN66_RS14920 (RFN66_14920) rsfS 2893416..2893772 (-) 357 WP_023855256.1 ribosome silencing factor -
  RFN66_RS14925 (RFN66_14925) yqeK 2893786..2894349 (-) 564 WP_025811126.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  RFN66_RS14930 (RFN66_14930) - 2894339..2894908 (-) 570 WP_025811125.1 nicotinate-nucleotide adenylyltransferase -
  RFN66_RS14935 (RFN66_14935) yhbY 2894927..2895217 (-) 291 WP_003183704.1 ribosome assembly RNA-binding protein YhbY -
  RFN66_RS14940 (RFN66_14940) aroE 2895211..2896047 (-) 837 WP_020452280.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22628.43 Da        Isoelectric Point: 4.6492

>NTDB_id=875921 RFN66_RS14905 WP_096748238.1 2891060..2891689(-) (comEA) [Bacillus paralicheniformis strain CP47]
MTDWLKQYKWHAAGGVALVLIISAAFMLLSGKRETSSGFSIPEEASAQTFDKKEEVKREKNAGEEEVIIDLKGAVKNPGV
YQMKEGDRVHDALKKAGGTEKKADQKQINLAAVLRDGMVLYIPFEGEEAAGSLSEADSRADGSSSDIVNINTASSEELQT
IPGIGPSKAEAVVEYREENGMFQTIEDITNVSGIGEKSFERIKSSITVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=875921 RFN66_RS14905 WP_096748238.1 2891060..2891689(-) (comEA) [Bacillus paralicheniformis strain CP47]
TTGACAGATTGGCTAAAACAATATAAATGGCATGCGGCGGGAGGTGTGGCACTCGTCTTGATCATCAGCGCTGCCTTCAT
GCTGTTAAGCGGAAAGCGTGAAACTTCATCCGGCTTCTCCATTCCTGAAGAGGCTTCTGCACAGACTTTTGACAAGAAAG
AGGAGGTGAAGCGGGAAAAAAACGCGGGGGAAGAGGAGGTTATCATCGATTTGAAAGGCGCTGTGAAAAATCCGGGCGTC
TATCAAATGAAGGAGGGAGACAGGGTGCACGATGCATTGAAAAAAGCAGGCGGCACCGAGAAAAAAGCGGACCAAAAGCA
GATTAATCTGGCAGCCGTTTTGCGGGACGGTATGGTTCTTTACATTCCATTTGAAGGGGAAGAGGCCGCTGGCTCTCTTT
CGGAAGCGGACTCAAGAGCAGACGGCAGCTCAAGTGATATCGTCAATATCAATACGGCTTCCTCTGAGGAGCTTCAGACG
ATTCCCGGGATCGGTCCTTCAAAAGCGGAAGCGGTAGTCGAATACCGCGAGGAGAACGGAATGTTTCAGACGATTGAAGA
CATAACAAACGTTTCTGGAATTGGAGAGAAGTCTTTTGAAAGAATAAAATCTTCAATCACGGTAAAGTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(69-122)

Helix-hairpin-helix motif (HHH_3)

(145-207)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

52.336

100

0.536
  comEA Staphylococcus aureus MW2

36.364

100

0.383
  comEA Staphylococcus aureus N315

35.909

100

0.378
  comEA Lactococcus lactis subsp. cremoris KW2

35.023

100

0.364