Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   AABK23_RS09875 Genome accession   NZ_AP027576
Coordinates   2069088..2070098 (+) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli strain LR-62-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2064088..2075098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABK23_RS09845 (VEE29_18800) nudB 2065812..2066264 (+) 453 WP_077248299.1 dihydroneopterin triphosphate diphosphatase -
  AABK23_RS09850 (VEE29_18810) yebC 2066293..2067033 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  AABK23_RS09855 (VEE29_18820) ruvC 2067068..2067589 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  AABK23_RS09860 (VEE29_18830) yebB 2067591..2068193 (-) 603 WP_022645808.1 YebB family permuted papain-like enzyme -
  AABK23_RS09865 yobI 2068264..2068329 (+) 66 WP_001386853.1 stress response small protein YobI -
  AABK23_RS09870 (VEE29_18840) ruvA 2068468..2069079 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  AABK23_RS09875 (VEE29_18850) ruvB 2069088..2070098 (+) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  AABK23_RS09880 (VEE29_18860) znuB 2070245..2071030 (-) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  AABK23_RS09885 (VEE29_18870) znuC 2071027..2071782 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  AABK23_RS09890 (VEE29_18880) znuA 2071861..2072793 (+) 933 WP_001347089.1 zinc ABC transporter substrate-binding protein ZnuA -
  AABK23_RS09895 (VEE29_18890) mepM 2072809..2074131 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=87591 AABK23_RS09875 WP_000568522.1 2069088..2070098(+) (ruvB) [Escherichia coli strain LR-62-2]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=87591 AABK23_RS09875 WP_000568522.1 2069088..2070098(+) (ruvB) [Escherichia coli strain LR-62-2]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAACCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCTAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGGGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTAGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCACGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGACTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCTGATATCGCTGCTCAGGCGCTGGATATGCTGAATGTCGATGCCGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCTGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCACCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494

Multiple sequence alignment