Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   RFF16_RS10640 Genome accession   NZ_CP133642
Coordinates   2246416..2247750 (+) Length   444 a.a.
NCBI ID   WP_010907082.1    Uniprot ID   Q9CLK3
Organism   Pasteurella multocida strain Past3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2241416..2252750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF16_RS10615 (RFF16_10610) - 2241492..2244053 (-) 2562 WP_010907084.1 penicillin-binding protein 1A -
  RFF16_RS10620 (RFF16_10615) - 2244189..2244962 (+) 774 WP_010907083.1 pilus assembly protein PilM -
  RFF16_RS10625 (RFF16_10620) - 2245002..2245517 (+) 516 WP_005751904.1 hypothetical protein -
  RFF16_RS10630 (RFF16_10625) - 2245517..2246041 (+) 525 WP_005754754.1 hypothetical protein -
  RFF16_RS10635 (RFF16_10630) - 2246044..2246406 (+) 363 WP_005748555.1 pilus assembly protein PilP -
  RFF16_RS10640 (RFF16_10635) comE 2246416..2247750 (+) 1335 WP_010907082.1 type IV pilus secretin PilQ Machinery gene
  RFF16_RS10645 (RFF16_10640) aroK 2247933..2248460 (+) 528 WP_005717623.1 shikimate kinase AroK -
  RFF16_RS10650 (RFF16_10645) aroB 2248477..2249565 (+) 1089 WP_010907081.1 3-dehydroquinate synthase -
  RFF16_RS10655 (RFF16_10650) - 2249569..2250474 (+) 906 WP_005723696.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  RFF16_RS10660 (RFF16_10655) - 2250521..2250826 (-) 306 WP_005723691.1 DUF721 domain-containing protein -
  RFF16_RS10665 (RFF16_10660) secM 2250919..2251230 (+) 312 WP_005723689.1 secA translation cis-regulator SecM -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49206.63 Da        Isoelectric Point: 8.0315

>NTDB_id=875455 RFF16_RS10640 WP_010907082.1 2246416..2247750(+) (comE) [Pasteurella multocida strain Past3]
MWRAFRKISLVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IARMKQLELHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLHFAKAAEVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQKTERHQKRELVIFVTPHIIKPSQGSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=875455 RFF16_RS10640 WP_010907082.1 2246416..2247750(+) (comE) [Pasteurella multocida strain Past3]
ATGTGGCGAGCATTCAGAAAAATATCTCTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCGCCTTTAGTCGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCACTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAGAATGAAACAACTTGAGTTACATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCCGCAGAGGTGGTGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
TAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCAGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACGG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTAGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGTGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACTCCTCATATTATCAAACCTAGCCA
AGGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(264-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9CLK3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

62.212

97.748

0.608
  comE Haemophilus influenzae 86-028NP

61.521

97.748

0.601
  comE Glaesserella parasuis strain SC1401

50.976

92.342

0.471
  pilQ Vibrio campbellii strain DS40M4

39.524

94.595

0.374
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.806

92.793

0.369
  pilQ Vibrio cholerae strain A1552

39.806

92.793

0.369