Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   RFF20_RS10140 Genome accession   NZ_CP133639
Coordinates   2152704..2154038 (+) Length   444 a.a.
NCBI ID   WP_104227536.1    Uniprot ID   -
Organism   Pasteurella multocida strain Past6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2147704..2159038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF20_RS10115 (RFF20_10115) - 2147780..2150341 (-) 2562 WP_324006036.1 penicillin-binding protein 1A -
  RFF20_RS10120 (RFF20_10120) - 2150477..2151250 (+) 774 WP_126417843.1 pilus assembly protein PilM -
  RFF20_RS10125 (RFF20_10125) - 2151290..2151805 (+) 516 WP_104884245.1 competence protein ComB -
  RFF20_RS10130 (RFF20_10130) - 2151805..2152329 (+) 525 WP_104227537.1 ATPase -
  RFF20_RS10135 (RFF20_10135) - 2152332..2152694 (+) 363 WP_083003380.1 pilus assembly protein PilP -
  RFF20_RS10140 (RFF20_10140) comE 2152704..2154038 (+) 1335 WP_104227536.1 type IV pilus secretin PilQ Machinery gene
  RFF20_RS10145 (RFF20_10145) aroK 2154221..2154748 (+) 528 WP_005717623.1 shikimate kinase AroK -
  RFF20_RS10150 (RFF20_10150) aroB 2154765..2155853 (+) 1089 WP_083003375.1 3-dehydroquinate synthase -
  RFF20_RS10155 (RFF20_10155) - 2155857..2156762 (+) 906 WP_250262986.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  RFF20_RS10160 (RFF20_10160) - 2156809..2157114 (-) 306 WP_083003370.1 DUF721 domain-containing protein -
  RFF20_RS10165 (RFF20_10165) secM 2157207..2157518 (+) 312 WP_005723689.1 secA translation cis-regulator SecM -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49192.51 Da        Isoelectric Point: 7.1842

>NTDB_id=875433 RFF20_RS10140 WP_104227536.1 2152704..2154038(+) (comE) [Pasteurella multocida strain Past6]
MWRAFRKLSFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IAKMKQLEFHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLHFAKAAEVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQETERHQKRELVIFVTPHIIKPTQSSPEQKTTGVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=875433 RFF20_RS10140 WP_104227536.1 2152704..2154038(+) (comE) [Pasteurella multocida strain Past6]
ATGTGGCGAGCATTCAGAAAACTATCTTTTGTGTACTTTTTATGTGGAGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCACCTTTAGTCGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAAAATGAAACAACTTGAATTCCATCAAGAAGGGAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCCGCAGAGGTGGTGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
CAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCGGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACGG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTAGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAGGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGGAAACTGAACGTCATCAAAAGCGAGAATTAGTGATTTTTGTCACGCCTCATATTATCAAACCGACCCA
AAGTTCGCCTGAACAAAAAACAACGGGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)

Bacterial type II and III secretion system protein (Secretin)

(264-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

61.573

100

0.617
  comE Haemophilus influenzae 86-028NP

60.899

100

0.61
  comE Glaesserella parasuis strain SC1401

48.611

97.297

0.473
  pilQ Vibrio campbellii strain DS40M4

39.524

94.595

0.374
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.806

92.793

0.369
  pilQ Vibrio cholerae strain A1552

39.806

92.793

0.369