Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   RFF38_RS11380 Genome accession   NZ_CP133638
Coordinates   2356290..2357624 (+) Length   444 a.a.
NCBI ID   WP_115300061.1    Uniprot ID   -
Organism   Pasteurella multocida strain Past9     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2351290..2362624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF38_RS11355 (RFF38_11355) - 2351366..2353927 (-) 2562 WP_005754757.1 penicillin-binding protein 1A -
  RFF38_RS11360 (RFF38_11360) - 2354063..2354836 (+) 774 WP_115300057.1 pilus assembly protein PilM -
  RFF38_RS11365 (RFF38_11365) - 2354876..2355391 (+) 516 WP_104884245.1 competence protein ComB -
  RFF38_RS11370 (RFF38_11370) - 2355391..2355915 (+) 525 WP_115300059.1 ATPase -
  RFF38_RS11375 (RFF38_11375) - 2355918..2356280 (+) 363 WP_115300060.1 pilus assembly protein PilP -
  RFF38_RS11380 (RFF38_11380) comE 2356290..2357624 (+) 1335 WP_115300061.1 type IV pilus secretin PilQ Machinery gene
  RFF38_RS11385 (RFF38_11385) aroK 2357807..2358334 (+) 528 WP_005717623.1 shikimate kinase AroK -
  RFF38_RS11390 (RFF38_11390) aroB 2358351..2359439 (+) 1089 WP_083003375.1 3-dehydroquinate synthase -
  RFF38_RS11395 (RFF38_11395) - 2359443..2360348 (+) 906 WP_108511556.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  RFF38_RS11400 (RFF38_11400) - 2360394..2360699 (-) 306 WP_083003370.1 DUF721 domain-containing protein -
  RFF38_RS11405 (RFF38_11405) secM 2360792..2361106 (+) 315 WP_108511555.1 secA translation cis-regulator SecM -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49304.73 Da        Isoelectric Point: 8.0309

>NTDB_id=875410 RFF38_RS11380 WP_115300061.1 2356290..2357624(+) (comE) [Pasteurella multocida strain Past9]
MWRAFRKLSFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IAKMKQLEFHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLHFAKAAEVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFTTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQKTERHQKRELVIFVTPHIIKPTQSSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=875410 RFF38_RS11380 WP_115300061.1 2356290..2357624(+) (comE) [Pasteurella multocida strain Past9]
ATGTGGCGAGCATTCAGAAAACTATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCACCTTTAGTCGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAAAATGAAACAACTTGAATTCCATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCGGCAAA
CGATACTCATCATCCTACATCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCCGCAGAGGTGGTGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
CAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCGGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTTACGACCAATAGC
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACGG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCAGTGGAATTTCGAGAAGCTGTATTAGGTTTAGATGTG
ACACCGCATATCTCAAAAGATAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAGGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACGCCTCATATTATCAAACCGACCCA
AAGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)

Bacterial type II and III secretion system protein (Secretin)

(264-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

62.471

96.622

0.604
  comE Haemophilus influenzae 86-028NP

61.201

97.523

0.597
  comE Glaesserella parasuis strain SC1401

48.611

97.297

0.473
  pilQ Vibrio campbellii strain DS40M4

39.524

94.595

0.374
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.806

92.793

0.369
  pilQ Vibrio cholerae strain A1552

39.806

92.793

0.369