Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   RFF39_RS10640 Genome accession   NZ_CP133634
Coordinates   2248100..2249434 (+) Length   444 a.a.
NCBI ID   WP_323997688.1    Uniprot ID   -
Organism   Pasteurella multocida strain Past33     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2243100..2254434
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF39_RS10615 (RFF39_10615) - 2243176..2245737 (-) 2562 WP_016534629.1 penicillin-binding protein 1A -
  RFF39_RS10620 (RFF39_10620) - 2245873..2246646 (+) 774 WP_323997683.1 competence protein ComA -
  RFF39_RS10625 (RFF39_10625) - 2246686..2247201 (+) 516 WP_005723706.1 hypothetical protein -
  RFF39_RS10630 (RFF39_10630) - 2247201..2247725 (+) 525 WP_005751903.1 hypothetical protein -
  RFF39_RS10635 (RFF39_10635) - 2247728..2248090 (+) 363 WP_323997686.1 pilus assembly protein PilP -
  RFF39_RS10640 (RFF39_10640) comE 2248100..2249434 (+) 1335 WP_323997688.1 type IV pilus secretin PilQ Machinery gene
  RFF39_RS10645 (RFF39_10645) aroK 2249617..2250144 (+) 528 WP_005717623.1 shikimate kinase AroK -
  RFF39_RS10650 (RFF39_10650) aroB 2250161..2251249 (+) 1089 WP_323997690.1 3-dehydroquinate synthase -
  RFF39_RS10655 (RFF39_10655) - 2251253..2252158 (+) 906 WP_005723696.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  RFF39_RS10660 (RFF39_10660) - 2252205..2252510 (-) 306 WP_005723691.1 DUF721 domain-containing protein -
  RFF39_RS10665 (RFF39_10665) secM 2252603..2252914 (+) 312 WP_005723689.1 secA translation cis-regulator SecM -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49284.74 Da        Isoelectric Point: 8.0315

>NTDB_id=875364 RFF39_RS10640 WP_323997688.1 2248100..2249434(+) (comE) [Pasteurella multocida strain Past33]
MWRAFRKISFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IARMKQLELHQEGKSYYLTSPSGVAVNDTHHPTSLMTSSIKLHFAKAAEVVKSLTSGQGSLLTVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNSILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQKTERHQKRELVIFVTPHIIKLSQGSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=875364 RFF39_RS10640 WP_323997688.1 2248100..2249434(+) (comE) [Pasteurella multocida strain Past33]
ATGTGGCGAGCATTCAGAAAAATATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCGCCTTTAGTCGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAGAATGAAACAACTTGAGTTACATCAAGAAGGGAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCAGTAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCTGCAGAGGTAGTGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTACTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
TAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCAGGAAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACGG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTGGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACTCGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTTGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAAAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACTCCTCATATTATCAAACTTAGCCA
AGGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCAAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(264-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

62.238

96.622

0.601
  comE Haemophilus influenzae 86-028NP

61.538

96.622

0.595
  comE Glaesserella parasuis strain SC1401

48.73

97.523

0.475
  pilQ Vibrio campbellii strain DS40M4

39.286

94.595

0.372
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.32

92.793

0.365
  pilQ Vibrio cholerae strain A1552

39.32

92.793

0.365