Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   RFF25_RS10430 Genome accession   NZ_CP133633
Coordinates   2198520..2199854 (+) Length   444 a.a.
NCBI ID   WP_250190859.1    Uniprot ID   -
Organism   Pasteurella multocida strain Pm1618     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2193520..2204854
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF25_RS10405 (RFF25_10400) - 2193596..2196157 (-) 2562 WP_250190858.1 penicillin-binding protein 1A -
  RFF25_RS10410 (RFF25_10405) - 2196293..2197066 (+) 774 WP_083003387.1 pilus assembly protein PilM -
  RFF25_RS10415 (RFF25_10410) - 2197106..2197621 (+) 516 WP_083003385.1 competence protein ComB -
  RFF25_RS10420 (RFF25_10415) - 2197621..2198145 (+) 525 WP_083003382.1 ATPase -
  RFF25_RS10425 (RFF25_10420) - 2198148..2198510 (+) 363 WP_083003380.1 pilus assembly protein PilP -
  RFF25_RS10430 (RFF25_10425) comE 2198520..2199854 (+) 1335 WP_250190859.1 type IV pilus secretin PilQ Machinery gene
  RFF25_RS10435 (RFF25_10430) aroK 2200037..2200564 (+) 528 WP_005717623.1 shikimate kinase AroK -
  RFF25_RS10440 (RFF25_10435) aroB 2200581..2201669 (+) 1089 WP_083003375.1 3-dehydroquinate synthase -
  RFF25_RS10445 (RFF25_10440) - 2201673..2202578 (+) 906 WP_083003372.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  RFF25_RS10450 (RFF25_10445) - 2202625..2202930 (-) 306 WP_083003370.1 DUF721 domain-containing protein -
  RFF25_RS10455 (RFF25_10450) secM 2203023..2203334 (+) 312 WP_005723689.1 secA translation cis-regulator SecM -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49266.68 Da        Isoelectric Point: 8.0308

>NTDB_id=875342 RFF25_RS10430 WP_250190859.1 2198520..2199854(+) (comE) [Pasteurella multocida strain Pm1618]
MWRAFRKLSFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IAKMKQLEFHQEGKSYYLTSSSGVAANDTHHPTSLMTSSIKLHFAKAADVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFTTNS
ATVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQETERHQKRELVIFVTPHIIKPSQGLPKQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=875342 RFF25_RS10430 WP_250190859.1 2198520..2199854(+) (comE) [Pasteurella multocida strain Pm1618]
ATGTGGCGAGCATTCAGAAAACTATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCACCTTTAGTCGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAAAATGAAACAACTGGAATTCCATCAAGAAGGGAAATCCTATTATTTAACTTCCTCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCCGCAGATGTAGTGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
CAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCGGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTTACGACCAATAGT
GCAACTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTAGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACGG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTAGAATTTCGAGAAGCTGTATTAGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTACCTTTGCTTAAACAT
GTGTTTAGTCAAGAAACTGAACGCCATCAAAAGCGGGAATTAGTGATTTTTGTCACGCCTCATATTATCAAACCTAGCCA
AGGTTTGCCTAAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCAAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(264-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

61.451

99.324

0.61
  comE Haemophilus influenzae 86-028NP

60.771

99.324

0.604
  comE Glaesserella parasuis strain SC1401

48.73

97.523

0.475
  pilQ Vibrio campbellii strain DS40M4

39.762

94.595

0.376
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.049

92.793

0.372
  pilQ Vibrio cholerae strain A1552

40.049

92.793

0.372