Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   RFF30_RS10385 Genome accession   NZ_CP133632
Coordinates   2220354..2221688 (+) Length   444 a.a.
NCBI ID   WP_324007136.1    Uniprot ID   -
Organism   Pasteurella multocida strain Pm1621     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2215354..2226688
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF30_RS10360 (RFF30_10360) - 2215430..2217991 (-) 2562 WP_005754757.1 penicillin-binding protein 1A -
  RFF30_RS10365 (RFF30_10365) - 2218127..2218900 (+) 774 WP_324007134.1 competence protein ComA -
  RFF30_RS10370 (RFF30_10370) - 2218940..2219455 (+) 516 WP_324007135.1 competence protein ComB -
  RFF30_RS10375 (RFF30_10375) - 2219455..2219979 (+) 525 WP_324007574.1 ATPase -
  RFF30_RS10380 (RFF30_10380) - 2219982..2220344 (+) 363 WP_005748555.1 pilus assembly protein PilP -
  RFF30_RS10385 (RFF30_10385) comE 2220354..2221688 (+) 1335 WP_324007136.1 type IV pilus secretin PilQ Machinery gene
  RFF30_RS10390 (RFF30_10390) aroK 2221871..2222398 (+) 528 WP_005717623.1 shikimate kinase AroK -
  RFF30_RS10395 (RFF30_10395) aroB 2222415..2223503 (+) 1089 WP_324007137.1 3-dehydroquinate synthase -
  RFF30_RS10400 (RFF30_10400) - 2223507..2224412 (+) 906 WP_005723696.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  RFF30_RS10405 (RFF30_10405) - 2224459..2224764 (-) 306 WP_005723691.1 DUF721 domain-containing protein -
  RFF30_RS10410 (RFF30_10410) secM 2224857..2225168 (+) 312 WP_005723689.1 secA translation cis-regulator SecM -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49242.66 Da        Isoelectric Point: 8.0309

>NTDB_id=875319 RFF30_RS10385 WP_324007136.1 2220354..2221688(+) (comE) [Pasteurella multocida strain Pm1621]
MWRAFRKISFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IAKMKQLELHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLHFAKAAEVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
TPVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQKTERHQKRELVIFVTPHIIKPSQGSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=875319 RFF30_RS10385 WP_324007136.1 2220354..2221688(+) (comE) [Pasteurella multocida strain Pm1621]
ATGTGGCGAGCATTCAGAAAAATATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCGCCTTTAGTCGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAAAATGAAACAACTTGAGTTGCATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCTAAAGCCGCAGAGGTGGTGAAATCTT
TAACTTCAGGACAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
TAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCGGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
ACACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAGGGGACAG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTAGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACTCCTCATATTATCAAACCTAGCCA
AGGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)

Bacterial type II and III secretion system protein (Secretin)

(264-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

62.5

97.297

0.608
  comE Haemophilus influenzae 86-028NP

61.806

97.297

0.601
  comE Glaesserella parasuis strain SC1401

49.192

97.523

0.48
  pilQ Vibrio campbellii strain DS40M4

39.524

94.595

0.374
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.563

92.793

0.367
  pilQ Vibrio cholerae strain A1552

39.563

92.793

0.367