Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   RE395_RS16565 Genome accession   NZ_CP133579
Coordinates   3587344..3588402 (-) Length   352 a.a.
NCBI ID   WP_010484061.1    Uniprot ID   A0A0L8AA94
Organism   Stenotrophomonas geniculata strain G303     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3582344..3593402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RE395_RS16545 - 3583273..3585246 (-) 1974 WP_019659995.1 type IV pilus secretin PilQ -
  RE395_RS16550 - 3585268..3585774 (-) 507 WP_010484064.1 pilus assembly protein PilP -
  RE395_RS16555 - 3585801..3586457 (-) 657 WP_010484063.1 type 4a pilus biogenesis protein PilO -
  RE395_RS16560 - 3586454..3587344 (-) 891 WP_010484062.1 PilN domain-containing protein -
  RE395_RS16565 pilM 3587344..3588402 (-) 1059 WP_010484061.1 pilus assembly protein PilM Machinery gene
  RE395_RS16570 - 3588622..3591045 (+) 2424 WP_419965242.1 penicillin-binding protein 1A -
  RE395_RS16575 - 3591280..3591924 (+) 645 WP_010484059.1 hypothetical protein -
  RE395_RS16580 - 3592101..3592844 (-) 744 WP_049432669.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37753.41 Da        Isoelectric Point: 4.4089

>NTDB_id=875061 RE395_RS16565 WP_010484061.1 3587344..3588402(-) (pilM) [Stenotrophomonas geniculata strain G303]
MGLIPKSQSPLVGVDISSTAVKLLQLSRSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAMNRSGSKAKLAAAA
VAGSAVITKVIPMPAELDENDMEAQIELEAVNYIPYPIEEVNLDFEVIGAIPNNPEMVQVLLAASRSENVELRQSALELG
GLQAKVMDVEAFAVENAYALVASELPVSIEGVVALVDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEIMRRYGLSYEE
AGLAKRQGGLPESYEMEVLEPFKEATVQQISRLLQFFYAGSEFNRVDHIVLAGGCAVLGGLPEMVEEQLGVPTVVANPLA
QMTLGPKVNAHALAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=875061 RE395_RS16565 WP_010484061.1 3587344..3588402(-) (pilM) [Stenotrophomonas geniculata strain G303]
GTGGGGCTCATCCCAAAAAGTCAGTCGCCGCTCGTAGGCGTCGACATCAGTTCGACTGCGGTAAAGCTTTTGCAGCTGTC
CCGCAGTGGCAACCGTTTTCGTGTGGAACATTACGCCGTGGAACCTCTTCCGCCAAACGCGGTGGTGGAGAAGAACATCG
TGGAAGTGGAGGCCGTGGGTGAGGCCATCCGCCGTGCGATGAACCGCTCGGGGAGCAAGGCCAAGCTGGCCGCTGCCGCC
GTCGCCGGTTCGGCGGTGATCACCAAGGTGATCCCGATGCCGGCCGAGCTCGACGAAAACGACATGGAAGCCCAGATCGA
GCTGGAAGCGGTCAACTACATTCCGTACCCGATCGAGGAAGTGAACCTGGACTTCGAGGTGATCGGGGCGATCCCGAACA
ACCCGGAGATGGTCCAGGTGCTGCTGGCCGCGTCGCGTTCGGAGAACGTGGAACTGCGCCAGTCGGCCCTGGAACTGGGT
GGCCTGCAGGCCAAGGTGATGGACGTGGAGGCCTTCGCGGTCGAGAACGCCTACGCCCTGGTGGCCAGCGAACTGCCGGT
GTCCATCGAGGGCGTGGTCGCGCTGGTCGACATCGGCGCCACCATGACCACCCTCAACGTCCTGCGCGGCGGCCGCAGCC
TGTACAGCCGCGAACAGGTGTTCGGTGGCAAGCAGCTGACCGACGAGATCATGCGCCGTTACGGCCTGAGCTACGAGGAG
GCCGGCCTGGCCAAGCGCCAGGGCGGGCTGCCGGAAAGCTACGAGATGGAAGTGCTGGAGCCGTTCAAGGAAGCCACGGT
CCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTACGCAGGCAGCGAGTTCAACCGCGTTGACCACATCGTGCTGGCCGGCG
GCTGCGCCGTGCTCGGTGGCCTGCCGGAAATGGTCGAGGAACAGCTGGGCGTGCCGACCGTGGTCGCCAACCCGCTGGCG
CAGATGACCCTGGGGCCGAAGGTGAACGCACATGCGCTGGCCCAGGATGCCCCCGCGCTGATGATCGCCACCGGTCTGGC
GCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0L8AA94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.136

100

0.511
  comM Acinetobacter nosocomialis M2

51.136

100

0.511
  comM Acinetobacter baylyi ADP1

50

100

0.5
  pilM Legionella pneumophila strain ERS1305867

47.578

99.716

0.474