Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RB215_RS12690 Genome accession   NZ_CP133571
Coordinates   2714402..2715442 (-) Length   346 a.a.
NCBI ID   WP_041454575.1    Uniprot ID   A0A0U2X9Z1
Organism   Pseudoalteromonas sp. HL-AS2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2709402..2720442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RB215_RS12660 (RB215_12660) - 2709738..2710217 (+) 480 WP_309216516.1 hypothetical protein -
  RB215_RS12665 (RB215_12665) rsmE 2710290..2711024 (+) 735 WP_011329246.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RB215_RS12670 (RB215_12670) gshB 2711079..2712029 (+) 951 WP_089368758.1 glutathione synthase -
  RB215_RS12675 (RB215_12675) - 2712170..2712727 (+) 558 WP_058374183.1 YqgE/AlgH family protein -
  RB215_RS12680 (RB215_12680) ruvX 2712740..2713189 (+) 450 WP_011329249.1 Holliday junction resolvase RuvX -
  RB215_RS12685 (RB215_12685) pilU 2713256..2714392 (-) 1137 WP_011329250.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RB215_RS12690 (RB215_12690) pilT 2714402..2715442 (-) 1041 WP_041454575.1 type IV pilus twitching motility protein PilT Machinery gene
  RB215_RS12695 (RB215_12695) - 2715445..2716158 (+) 714 WP_011329252.1 YggS family pyridoxal phosphate-dependent enzyme -
  RB215_RS12700 (RB215_12700) proC 2716176..2716997 (+) 822 WP_011329253.1 pyrroline-5-carboxylate reductase -
  RB215_RS12705 (RB215_12705) - 2717010..2717543 (+) 534 WP_058374186.1 YggT family protein -
  RB215_RS12710 (RB215_12710) - 2717602..2718045 (+) 444 WP_182676591.1 DUF4426 domain-containing protein -
  RB215_RS12715 (RB215_12715) rdgB 2718053..2718652 (+) 600 WP_309216517.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  RB215_RS12720 (RB215_12720) hemW 2718649..2719785 (+) 1137 WP_011329257.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38170.55 Da        Isoelectric Point: 6.5036

>NTDB_id=874993 RB215_RS12690 WP_041454575.1 2714402..2715442(-) (pilT) [Pseudoalteromonas sp. HL-AS2]
MDITELLAFSVQHKASDLHLSSGVSPMIRVDGDVRRINIPALGDKDVNNLVYDIMNDNQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNTHRGPAAVFRTIPSEVLTLDDLGAPDIFKTIADNPRGLVLVTGPTGSGKSTTLAAMVDYINQTKHHHILTIED
PIEFVHDNKLSLINQREVHRDTHSFKNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKAMIRSMLSESLRAVISQTLLKKIGGGRVAAHEIMLAVPAIRNLIREDKVAQMYSSIQTGASHGMQTMDQCL
TNLVNHGIVTNIAAQAKAQDKTQFGG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=874993 RB215_RS12690 WP_041454575.1 2714402..2715442(-) (pilT) [Pseudoalteromonas sp. HL-AS2]
ATGGATATTACCGAATTATTGGCGTTTAGTGTGCAACACAAAGCATCCGATTTACATTTATCATCAGGGGTTTCACCTAT
GATACGCGTAGACGGTGATGTGCGGCGTATTAATATACCAGCACTAGGAGACAAAGATGTAAACAACCTAGTTTACGATA
TTATGAACGATAACCAGCGCAAGGACTACGAACAAAATCTCGAAGTGGATTTTTCATTTGAAGTGCCTAATTTAGCACGT
TTTCGTGTTAATGCCTTTAATACTCATCGTGGCCCAGCCGCTGTATTTCGTACGATCCCCAGTGAAGTGCTCACACTAGA
TGATTTAGGTGCTCCCGATATTTTTAAAACCATTGCCGATAATCCTCGCGGCTTAGTATTAGTAACTGGCCCTACAGGGT
CAGGTAAGTCGACAACATTAGCGGCTATGGTTGATTATATAAATCAAACAAAACATCACCATATTCTCACTATTGAAGAT
CCAATAGAGTTTGTCCACGATAACAAATTAAGTCTGATTAATCAGCGAGAAGTACATCGCGACACACATAGCTTTAAAAA
TGCGCTACGCAGTGCACTTCGTGAAGATCCAGATGTAATATTAGTTGGTGAATTACGTGATCTGGAAACCATTCGCCTGG
CAATGACAGCAGCCGAAACTGGTCACTTAGTATTTGGCACTTTGCACACCACCTCTGCACCTAAAACCATTGACCGTATT
ATAGATGTATTCCCAGGTGAAGAAAAAGCCATGATCCGCTCTATGCTCTCGGAATCATTACGTGCGGTAATTTCGCAAAC
CCTGCTTAAAAAAATTGGCGGTGGGCGAGTAGCTGCTCACGAAATTATGCTTGCAGTACCTGCTATTCGTAACTTAATTC
GTGAAGATAAAGTGGCGCAAATGTACTCGTCTATTCAAACTGGCGCTTCGCATGGAATGCAAACGATGGATCAATGTTTA
ACAAACTTAGTTAATCATGGCATTGTTACCAATATTGCAGCGCAAGCTAAAGCGCAAGACAAAACTCAGTTCGGTGGTTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0U2X9Z1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

75.581

99.422

0.751
  pilT Acinetobacter nosocomialis M2

75.581

99.422

0.751
  pilT Acinetobacter baumannii D1279779

75.581

99.422

0.751
  pilT Acinetobacter baumannii strain A118

75.581

99.422

0.751
  pilT Pseudomonas stutzeri DSM 10701

75.291

99.422

0.749
  pilT Pseudomonas aeruginosa PAK

74.709

99.422

0.743
  pilT Legionella pneumophila strain Lp02

72.093

99.422

0.717
  pilT Legionella pneumophila strain ERS1305867

72.093

99.422

0.717
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.271

97.977

0.708
  pilT Vibrio cholerae strain A1552

72.271

97.977

0.708
  pilT Neisseria gonorrhoeae MS11

70.674

98.555

0.697
  pilT Neisseria meningitidis 8013

70.381

98.555

0.694
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

99.711

0.529
  pilU Vibrio cholerae strain A1552

42.769

93.931

0.402
  pilU Pseudomonas stutzeri DSM 10701

39.577

95.665

0.379
  pilU Acinetobacter baylyi ADP1

39.634

94.798

0.376