Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   WHL52_RS13860 Genome accession   NZ_CP149576
Coordinates   2635734..2636351 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   P39694
Organism   Bacillus subtilis strain DSM 10     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2630734..2641351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHL52_RS13830 rpsT 2631036..2631302 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  WHL52_RS13835 holA 2631317..2632360 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  WHL52_RS13840 - 2632400..2632549 (-) 150 WP_003229985.1 hypothetical protein -
  WHL52_RS13845 yqzM 2632590..2632724 (+) 135 WP_003229983.1 YqzM family protein -
  WHL52_RS13850 comEC 2632764..2635094 (-) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  WHL52_RS13855 comEB 2635098..2635667 (-) 570 WP_003229978.1 ComE operon protein 2 -
  WHL52_RS13860 comEA 2635734..2636351 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  WHL52_RS13865 comER 2636435..2637256 (+) 822 WP_004398597.1 late competence protein ComER -
  WHL52_RS13870 yqeM 2637322..2638065 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  WHL52_RS13875 rsfS 2638062..2638418 (-) 357 WP_003229971.1 ribosome silencing factor -
  WHL52_RS13880 yqeK 2638436..2638996 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  WHL52_RS13885 nadD 2638986..2639555 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  WHL52_RS13890 yhbY 2639567..2639857 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  WHL52_RS13895 aroE 2639851..2640693 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=874983 WHL52_RS13860 WP_004398514.1 2635734..2636351(-) (comEA) [Bacillus subtilis strain DSM 10]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=874983 WHL52_RS13860 WP_004398514.1 2635734..2636351(-) (comEA) [Bacillus subtilis strain DSM 10]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterProScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 8DFK

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39