Detailed information NTG0874938

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RDV84_RS00900
• Genome accession: NZ_CP133568
• Coordinates: 183131..184297 (-)
• Length: 388 a.a.
• NCBI ID: WP_057946566.1
• Uniprot ID: A0A0S2DDC7
• Organism: Lysobacter yananisis strain SNNU513
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 178131..189297

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RDV84_RS00880 (RDV84_00880)	-	178752..179867 (-)	1116	WP_309152170.1	hypothetical protein	-
RDV84_RS00885 (RDV84_00885)	-	179864..181024 (-)	1161	WP_309152171.1	ATP-grasp domain-containing protein	-
RDV84_RS00890 (RDV84_00890)	-	181432..182337 (+)	906	WP_309152172.1	hypothetical protein	-
RDV84_RS00895 (RDV84_00895)	-	182462..183034 (-)	573	WP_309152173.1	hypothetical protein	-
RDV84_RS00900 (RDV84_00900)	pilU	183131..184297 (-)	1167	WP_057946566.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RDV84_RS00905 (RDV84_00905)	pilT	184410..185447 (-)	1038	WP_057946565.1	type IV pilus twitching motility protein PilT	Machinery gene
RDV84_RS00910 (RDV84_00910)	-	185627..186319 (+)	693	WP_309152174.1	YggS family pyridoxal phosphate-dependent enzyme	-
RDV84_RS00915 (RDV84_00915)	-	186350..186979 (-)	630	WP_309152175.1	HAD family hydrolase	-
RDV84_RS00920 (RDV84_00920)	proC	187099..187935 (+)	837	WP_309152176.1	pyrroline-5-carboxylate reductase	-
RDV84_RS00925 (RDV84_00925)	-	187932..188393 (+)	462	WP_309152177.1	DUF4426 domain-containing protein	-

Sequence

Protein

• Length: 388 a.a.
• Molecular weight: 43065.41 Da
• Isoelectric Point: 6.8286

>NTDB_id=874938 RDV84_RS00900 WP_057946566.1 183131..184297(-) (pilU) [Lysobacter yananisis strain SNNU513]
MNTPAPSNPTTASGAIDFTSFLKLMAHQRASDLFITAGMPPSMKVHGKITPITQNPLTPQQSRDLVLNVMSPQQREEFEK
THECNFAIGVTGVGRFRVSCFYQRNQVGMVLRRIETKIPSVDELNLPPIIKTLAMTKRGIIIFVGATGTGKSTSLAAMIG
YRNQNSTGHIITIEDPIEFVHRHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVL
CTLHANNANQAMDRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEILLGTPLAQDYIRDGEIHKLKEL
MKESTQLGMKTFDQALFELYQGGEISYEDALRYADSQNEVRLRIKLAQGGDARTLSQGLDGVEVAEVR

Nucleotide

• Length: 1167 bp

>NTDB_id=874938 RDV84_RS00900 WP_057946566.1 183131..184297(-) (pilU) [Lysobacter yananisis strain SNNU513]
ATGAACACGCCCGCCCCGTCCAACCCGACCACCGCCTCCGGCGCGATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCA
CCAGCGCGCCTCGGACTTGTTCATCACCGCCGGCATGCCGCCGTCGATGAAGGTGCACGGCAAGATCACCCCGATCACCC
AGAACCCGCTGACCCCGCAGCAGAGCCGCGACCTCGTCCTCAACGTGATGAGCCCGCAGCAGCGCGAGGAGTTCGAAAAG
ACCCACGAGTGCAACTTCGCCATCGGCGTCACCGGCGTCGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGT
CGGCATGGTGCTGCGCCGGATCGAGACCAAGATCCCCAGCGTCGACGAGCTGAACCTGCCGCCGATCATCAAGACGCTGG
CGATGACCAAGCGCGGCATCATCATCTTCGTCGGCGCCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGC
TACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAGGACCCGATCGAGTTCGTGCACCGCCACGAGGGCTGCAT
CATCACCCAGCGCGAAGTCGGCATCGACACCGACAGCTGGGAAGCCGCGCTCAAGAACACCCTGCGCCAGGCGCCGGACG
TGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCATGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTG
TGCACCCTGCACGCCAACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCCGAAGACCGCCGCAACCAGCT
GTTGATGGACTTGTCGCTGAACCTCAAGGGCGTGGTCGCCCAGCAGCTGATCCCGACCCCCGACGGCAAGGGCCGGCGCG
TGGCGATGGAGATCCTGCTCGGCACCCCGCTGGCGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGCTG
ATGAAGGAATCCACCCAGTTGGGCATGAAGACCTTCGACCAGGCCCTGTTCGAGCTCTACCAGGGCGGCGAGATCAGCTA
CGAGGACGCGCTGCGCTACGCCGACTCGCAGAACGAAGTGCGCCTGCGGATCAAGCTGGCGCAGGGCGGCGACGCGCGGA
CCTTGTCGCAGGGGTTGGATGGGGTGGAGGTGGCGGAGGTGCGGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (130-284)

3D structure

• AlphaFold DB: A0A0S2DDC7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	69.21	94.588	0.655
pilU	Acinetobacter baylyi ADP1	65.546	92.01	0.603
pilU	Vibrio cholerae strain A1552	53.09	91.753	0.487
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.595	90.464	0.376