Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   WJ399_RS29650 Genome accession   NZ_CP149513
Coordinates   6311077..6311673 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain 2023CK-01620     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6306077..6316673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJ399_RS29630 (WJ399_29630) aroK 6307208..6307726 (-) 519 WP_003095833.1 shikimate kinase AroK -
  WJ399_RS29635 (WJ399_29635) pilQ 6307738..6309882 (-) 2145 WP_023087871.1 type 4a pilus secretin PilQ Machinery gene
  WJ399_RS29640 (WJ399_29640) comL/pilP 6309936..6310460 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  WJ399_RS29645 (WJ399_29645) pilO 6310457..6311080 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  WJ399_RS29650 (WJ399_29650) pilN 6311077..6311673 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  WJ399_RS29655 (WJ399_29655) pilM 6311673..6312737 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  WJ399_RS29660 (WJ399_29660) - 6312922..6315393 (+) 2472 WP_003125627.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=874858 WJ399_RS29650 WP_003095839.1 6311077..6311673(-) (pilN) [Pseudomonas aeruginosa strain 2023CK-01620]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=874858 WJ399_RS29650 WP_003095839.1 6311077..6311673(-) (pilN) [Pseudomonas aeruginosa strain 2023CK-01620]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCTGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGTTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369