Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RDJ12_RS05265 Genome accession   NZ_CP133437
Coordinates   1124851..1125318 (-) Length   155 a.a.
NCBI ID   WP_261920343.1    Uniprot ID   A0A8E3MDR2
Organism   Mergibacter septicus strain 19Y0039     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1119851..1130318
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RDJ12_RS05235 (RDJ12_05235) - 1120251..1120523 (-) 273 WP_261920967.1 GNAT family N-acetyltransferase -
  RDJ12_RS05240 (RDJ12_05240) yacG 1120563..1120769 (-) 207 WP_261920348.1 DNA gyrase inhibitor YacG -
  RDJ12_RS05245 (RDJ12_05245) coaE 1120762..1121400 (-) 639 WP_261920347.1 dephospho-CoA kinase -
  RDJ12_RS05250 (RDJ12_05250) - 1121439..1122128 (-) 690 WP_261920346.1 prepilin peptidase -
  RDJ12_RS05255 (RDJ12_05255) - 1122170..1123396 (-) 1227 WP_261920345.1 type II secretion system F family protein -
  RDJ12_RS05260 (RDJ12_05260) pilB 1123411..1124829 (-) 1419 WP_261920344.1 GspE/PulE family protein Machinery gene
  RDJ12_RS05265 (RDJ12_05265) pilA 1124851..1125318 (-) 468 WP_261920343.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  RDJ12_RS05270 (RDJ12_05270) ampD 1125445..1126044 (+) 600 WP_373463079.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RDJ12_RS05275 (RDJ12_05275) - 1126207..1127274 (+) 1068 WP_261920342.1 LysM-like peptidoglycan-binding domain-containing protein -
  RDJ12_RS05280 (RDJ12_05280) cgtA 1127336..1128496 (-) 1161 WP_261920341.1 Obg family GTPase CgtA -
  RDJ12_RS05285 (RDJ12_05285) - 1128540..1129472 (-) 933 WP_261920340.1 DMT family transporter -
  RDJ12_RS05290 (RDJ12_05290) rpmA 1129644..1129901 (-) 258 WP_261920339.1 50S ribosomal protein L27 -
  RDJ12_RS05295 (RDJ12_05295) rplU 1129922..1130233 (-) 312 WP_261920338.1 50S ribosomal protein L21 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16212.65 Da        Isoelectric Point: 7.5827

>NTDB_id=874050 RDJ12_RS05265 WP_261920343.1 1124851..1125318(-) (pilA) [Mergibacter septicus strain 19Y0039]
MKRSTLTQAALSRVGLSQGFTLIELMIVIAIIAVLATVAIPSYQNYTKKAAMSELIQAASPYKAEVELCIYNSGSKTNCS
AGQNGIQAAVTESEKVKYLKTVGVNAGVISVTGKGTLENIGYTLTPNGGENGEVIDWAVNCTGDKGIFPVGFCAN

Nucleotide


Download         Length: 468 bp        

>NTDB_id=874050 RDJ12_RS05265 WP_261920343.1 1124851..1125318(-) (pilA) [Mergibacter septicus strain 19Y0039]
ATGAAAAGATCAACCTTAACTCAAGCAGCTTTATCTCGAGTTGGATTATCTCAAGGCTTTACTTTAATTGAATTGATGAT
TGTCATCGCAATCATTGCTGTATTAGCCACGGTGGCTATTCCTTCCTATCAAAACTATACCAAGAAAGCTGCAATGTCAG
AATTGATTCAAGCTGCTTCGCCGTATAAGGCTGAAGTTGAGTTATGTATTTATAATTCAGGTTCTAAGACAAATTGCTCA
GCAGGGCAAAATGGTATTCAAGCGGCGGTTACTGAGAGTGAGAAAGTAAAATATTTGAAAACGGTCGGAGTAAATGCAGG
TGTTATTTCAGTAACAGGTAAAGGAACATTGGAGAATATTGGATATACACTGACGCCAAATGGTGGCGAAAATGGAGAGG
TCATTGATTGGGCTGTAAACTGTACGGGGGATAAAGGAATATTTCCAGTAGGTTTTTGTGCTAATTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(17-39)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

55.396

89.677

0.497
  pilA Glaesserella parasuis strain SC1401

53.901

90.968

0.49
  pilA Haemophilus influenzae Rd KW20

53.957

89.677

0.484
  pilA Vibrio campbellii strain DS40M4

38.356

94.194

0.361