Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   RCG25_RS08275 Genome accession   NZ_CP133269
Coordinates   1676946..1677254 (+) Length   102 a.a.
NCBI ID   WP_308084128.1    Uniprot ID   -
Organism   Neobacillus sp. PS2-9     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1671946..1682254
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCG25_RS08250 (RCG25_08250) - 1672258..1673349 (+) 1092 WP_308083187.1 SAM-dependent methyltransferase -
  RCG25_RS08255 (RCG25_08255) - 1673574..1673816 (-) 243 WP_308083188.1 DUF2626 domain-containing protein -
  RCG25_RS08260 (RCG25_08260) - 1673900..1674598 (-) 699 WP_308083190.1 helix-turn-helix domain-containing protein -
  RCG25_RS08265 (RCG25_08265) comGA 1674828..1675910 (+) 1083 WP_308083191.1 competence type IV pilus ATPase ComGA Machinery gene
  RCG25_RS08270 (RCG25_08270) comGB 1675897..1676928 (+) 1032 WP_308083193.1 competence type IV pilus assembly protein ComGB -
  RCG25_RS08275 (RCG25_08275) comGC 1676946..1677254 (+) 309 WP_308084128.1 competence type IV pilus major pilin ComGC Machinery gene
  RCG25_RS08280 (RCG25_08280) comGD 1677254..1677694 (+) 441 WP_308083194.1 competence type IV pilus minor pilin ComGD -
  RCG25_RS08285 (RCG25_08285) comGE 1677678..1678016 (+) 339 WP_308083195.1 competence type IV pilus minor pilin ComGE -
  RCG25_RS08290 (RCG25_08290) comGF 1677964..1678440 (+) 477 WP_308083196.1 competence type IV pilus minor pilin ComGF -
  RCG25_RS08295 (RCG25_08295) comGG 1678437..1678820 (+) 384 WP_308083197.1 competence type IV pilus minor pilin ComGG -
  RCG25_RS08300 (RCG25_08300) - 1678899..1679084 (+) 186 WP_308083198.1 YqzE family protein -
  RCG25_RS08305 (RCG25_08305) - 1679323..1680114 (-) 792 WP_308083199.1 YqhG family protein -
  RCG25_RS08310 (RCG25_08310) - 1680092..1681780 (-) 1689 WP_308083200.1 SNF2-related protein -

Sequence

Protein


Download         Length: 102 a.a.        Molecular weight: 11213.09 Da        Isoelectric Point: 5.7782

>NTDB_id=873139 RCG25_RS08275 WP_308084128.1 1676946..1677254(+) (comGC) [Neobacillus sp. PS2-9]
MNNEKGFTLIEMMIVMLVISILLIITIPNVAKHNTNINNKGCEAYVKMVQAQVQAYEMDKNKVPTLQDLITEEYIKNDAK
GCPNGKTVSIDSSGNVTAVVTP

Nucleotide


Download         Length: 309 bp        

>NTDB_id=873139 RCG25_RS08275 WP_308084128.1 1676946..1677254(+) (comGC) [Neobacillus sp. PS2-9]
ATGAATAATGAAAAAGGGTTTACACTTATTGAAATGATGATAGTTATGCTAGTAATTTCAATCCTGTTGATCATCACAAT
TCCAAATGTAGCAAAACATAATACTAATATAAATAATAAGGGTTGCGAGGCTTATGTGAAAATGGTCCAAGCACAAGTTC
AAGCCTATGAAATGGATAAAAACAAGGTGCCAACACTGCAAGACCTTATAACCGAAGAGTATATAAAGAATGATGCAAAA
GGATGTCCGAACGGAAAAACAGTGAGTATAGATTCAAGTGGAAATGTTACAGCAGTAGTAACACCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

58.427

87.255

0.51
  comGC Staphylococcus aureus MW2

46.316

93.137

0.431
  comGC Staphylococcus aureus N315

46.316

93.137

0.431
  comGC/cglC Streptococcus pneumoniae TIGR4

40.217

90.196

0.363