Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RCG25_RS08265 Genome accession   NZ_CP133269
Coordinates   1674828..1675910 (+) Length   360 a.a.
NCBI ID   WP_308083191.1    Uniprot ID   -
Organism   Neobacillus sp. PS2-9     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1669828..1680910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCG25_RS08235 (RCG25_08235) - 1670702..1671019 (+) 318 WP_308083183.1 MTH1187 family thiamine-binding protein -
  RCG25_RS08240 (RCG25_08240) - 1671077..1671253 (-) 177 WP_308083184.1 DUF2759 domain-containing protein -
  RCG25_RS08245 (RCG25_08245) - 1671461..1672096 (+) 636 WP_308083186.1 MBL fold metallo-hydrolase -
  RCG25_RS08250 (RCG25_08250) - 1672258..1673349 (+) 1092 WP_308083187.1 SAM-dependent methyltransferase -
  RCG25_RS08255 (RCG25_08255) - 1673574..1673816 (-) 243 WP_308083188.1 DUF2626 domain-containing protein -
  RCG25_RS08260 (RCG25_08260) - 1673900..1674598 (-) 699 WP_308083190.1 helix-turn-helix domain-containing protein -
  RCG25_RS08265 (RCG25_08265) comGA 1674828..1675910 (+) 1083 WP_308083191.1 competence type IV pilus ATPase ComGA Machinery gene
  RCG25_RS08270 (RCG25_08270) comGB 1675897..1676928 (+) 1032 WP_308083193.1 competence type IV pilus assembly protein ComGB -
  RCG25_RS08275 (RCG25_08275) comGC 1676946..1677254 (+) 309 WP_308084128.1 competence type IV pilus major pilin ComGC Machinery gene
  RCG25_RS08280 (RCG25_08280) comGD 1677254..1677694 (+) 441 WP_308083194.1 competence type IV pilus minor pilin ComGD -
  RCG25_RS08285 (RCG25_08285) comGE 1677678..1678016 (+) 339 WP_308083195.1 competence type IV pilus minor pilin ComGE -
  RCG25_RS08290 (RCG25_08290) comGF 1677964..1678440 (+) 477 WP_308083196.1 competence type IV pilus minor pilin ComGF -
  RCG25_RS08295 (RCG25_08295) comGG 1678437..1678820 (+) 384 WP_308083197.1 competence type IV pilus minor pilin ComGG -
  RCG25_RS08300 (RCG25_08300) - 1678899..1679084 (+) 186 WP_308083198.1 YqzE family protein -
  RCG25_RS08305 (RCG25_08305) - 1679323..1680114 (-) 792 WP_308083199.1 YqhG family protein -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 40679.11 Da        Isoelectric Point: 8.6773

>NTDB_id=873138 RCG25_RS08265 WP_308083191.1 1674828..1675910(+) (comGA) [Neobacillus sp. PS2-9]
MVLIASSIELLANRIISDAARNQATDIHIIPRKKDTLVQIRLTNKLIPRLSLPKEECDRLISHFKFTANMDIGERRRPQS
GAIFCEVDGQLMGLRLSTLPSYNRESLVIRLLPQQEQIPFHQLSLFPSMTRKLLALLKHAHGLIIFTGPTGSGKTTTLYS
LLNETAHLFHRNVITLEDPIEKNYDSVLQVQVNEKAGVTYAAGLKAILRHDPDIIMVGEIRDAETAKIAVRAALTGHLVL
STMHTRDTKGAVYRLREFGVNWLEVEQTLIAVTAQRLVELTCPFCEGECSPNCYSYGRWKRASVFELLSGRNLHSVMKVA
RGEVMNPRYRTIKDVINKGIALGYIQESEYERLVYEDETT

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=873138 RCG25_RS08265 WP_308083191.1 1674828..1675910(+) (comGA) [Neobacillus sp. PS2-9]
GTGGTACTCATTGCAAGTTCAATTGAACTATTAGCTAATCGAATCATCTCAGATGCTGCTAGGAATCAGGCAACGGATAT
TCACATTATTCCAAGAAAGAAAGACACACTCGTCCAAATTCGTTTGACCAACAAACTCATTCCTCGACTCTCCCTTCCAA
AAGAAGAATGCGACAGATTAATTTCCCATTTTAAATTTACAGCCAATATGGATATCGGAGAAAGAAGGCGTCCGCAGAGC
GGAGCTATTTTTTGTGAGGTAGACGGACAATTGATGGGCCTCAGGCTTTCCACTTTACCATCTTATAATAGAGAGAGCCT
AGTCATCAGGCTTTTACCCCAACAAGAACAAATTCCCTTTCATCAACTCTCTTTATTTCCTTCTATGACTAGAAAGCTGT
TAGCCCTTCTCAAACACGCTCATGGATTAATTATTTTCACAGGTCCCACGGGTTCTGGCAAAACGACAACCCTCTACTCT
TTATTAAATGAAACGGCTCATTTATTCCATCGAAATGTGATTACACTTGAAGATCCTATTGAAAAAAATTATGATTCTGT
CCTCCAGGTCCAGGTCAATGAAAAAGCAGGAGTTACATATGCTGCAGGATTAAAGGCTATTCTACGCCACGACCCAGACA
TTATTATGGTTGGTGAGATAAGGGATGCAGAAACTGCAAAGATAGCTGTACGTGCTGCTTTAACAGGTCATTTAGTCCTT
TCTACCATGCATACCAGAGATACCAAGGGAGCCGTGTACCGTCTTCGAGAATTTGGTGTGAATTGGTTAGAAGTGGAACA
GACACTTATTGCTGTTACTGCACAAAGACTTGTTGAGCTTACCTGTCCGTTTTGCGAGGGTGAGTGTTCTCCGAATTGTT
ATTCGTATGGAAGGTGGAAACGAGCAAGTGTATTTGAACTCTTGTCAGGAAGGAATTTACATTCCGTTATGAAAGTGGCA
AGGGGAGAGGTGATGAATCCTAGGTACCGAACAATTAAGGATGTCATTAATAAAGGTATTGCATTAGGGTATATTCAAGA
ATCTGAATATGAACGACTGGTGTATGAAGATGAAACCACGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.374

98.056

0.553
  pilB Glaesserella parasuis strain SC1401

42.997

85.278

0.367
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.695

99.167

0.364