Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RCF98_RS05280 Genome accession   NZ_CP133218
Coordinates   1087557..1088735 (+) Length   392 a.a.
NCBI ID   WP_028488344.1    Uniprot ID   -
Organism   Thiothrix lacustris strain MK1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1082557..1093735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCF98_RS05250 (RCF98_05250) - 1082557..1082985 (+) 429 WP_308896647.1 hypothetical protein -
  RCF98_RS05255 (RCF98_05255) ald 1082982..1083968 (-) 987 WP_308896649.1 alanine dehydrogenase -
  RCF98_RS05260 (RCF98_05260) - 1083934..1084560 (-) 627 WP_308896651.1 DUF1461 domain-containing protein -
  RCF98_RS05265 (RCF98_05265) rpiA 1084553..1085209 (-) 657 WP_028488341.1 ribose-5-phosphate isomerase RpiA -
  RCF98_RS05270 (RCF98_05270) ilvA 1085303..1086817 (+) 1515 WP_028488342.1 threonine ammonia-lyase, biosynthetic -
  RCF98_RS05275 (RCF98_05275) - 1086809..1087501 (-) 693 WP_308896653.1 pseudouridine synthase -
  RCF98_RS05280 (RCF98_05280) pilU 1087557..1088735 (+) 1179 WP_028488344.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RCF98_RS05285 (RCF98_05285) argS 1088737..1090482 (-) 1746 WP_308896656.1 arginine--tRNA ligase -
  RCF98_RS05290 (RCF98_05290) yciA 1090604..1090999 (-) 396 WP_028488346.1 acyl-CoA thioester hydrolase YciA -
  RCF98_RS05295 (RCF98_05295) - 1091048..1092055 (-) 1008 WP_156946742.1 hypothetical protein -
  RCF98_RS05305 (RCF98_05305) dusA 1092242..1093210 (-) 969 WP_308896659.1 tRNA dihydrouridine(20/20a) synthase DusA -
  RCF98_RS05310 (RCF98_05310) - 1093296..1093490 (+) 195 WP_028488348.1 hypothetical protein -

Sequence

Protein


Download         Length: 392 a.a.        Molecular weight: 43731.58 Da        Isoelectric Point: 6.8299

>NTDB_id=872547 RCF98_RS05280 WP_028488344.1 1087557..1088735(+) (pilU) [Thiothrix lacustris strain MK1]
MKLKDYLRILAHYDGSDLYLTADLEPKAKFQGKLKALDKVVMTPEMLKDMAYELMNKEQQQQFEHKPEMNLAISEEGVGR
FRVNIFKQRHKIAMVIRNIKTDIPNADKLGLPQVLKDVIMEKRGLILFVGGTGSGKSTSLAALIDHRNASADGHIITIED
PVEYVHPHKKCIINQREVGVDTDSYEDALKNTLRQAPDVILIGEIRAQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRHQLLMDLSLNLKAFVSQRLIPTIDGKRVAAIEILLGTPMIRDLIMKGDVHAIKEIMEKSEEQGMQTFDSHL
YKLYLAGQISLAETLRNADSPSNLKLKINLSGNLAKPRPTAAAAAPEAKSTAASADFMANLSLEPKPEVELT

Nucleotide


Download         Length: 1179 bp        

>NTDB_id=872547 RCF98_RS05280 WP_028488344.1 1087557..1088735(+) (pilU) [Thiothrix lacustris strain MK1]
ATGAAACTCAAAGACTATCTGCGTATCCTCGCCCATTACGACGGTTCCGACCTGTACCTCACCGCCGATCTCGAACCCAA
AGCCAAATTCCAAGGCAAACTCAAAGCCCTCGATAAAGTCGTCATGACCCCAGAAATGCTCAAGGACATGGCTTACGAGC
TAATGAACAAGGAACAGCAGCAGCAGTTTGAACACAAACCCGAAATGAACCTCGCCATCAGCGAAGAAGGCGTTGGCCGT
TTCCGCGTCAATATTTTCAAGCAGCGCCACAAAATCGCGATGGTCATCCGCAATATCAAAACCGACATTCCCAACGCCGA
CAAACTCGGCCTGCCGCAAGTGCTCAAAGACGTGATCATGGAAAAACGTGGCTTGATCCTGTTCGTCGGCGGCACAGGTT
CCGGCAAATCGACTTCACTCGCCGCACTGATCGACCACCGCAATGCTTCGGCTGACGGGCATATCATCACCATCGAAGAC
CCGGTGGAATACGTGCATCCGCACAAAAAATGTATCATCAACCAGCGCGAAGTCGGCGTCGACACCGACAGCTACGAAGA
CGCGCTGAAAAATACCCTGCGCCAAGCGCCCGATGTGATCCTGATCGGCGAAATCCGCGCCCAAGAAACCATGGAACACG
CCCTAGCCTTCGCCGAAACAGGTCACCTGTGCCTTTCCACCCTGCACGCCAACAACGCCAACCAAGCGCTAGACCGCATC
ATCAACTTCTTCCCCGAAGAACGCCGCCACCAATTGCTGATGGATTTATCCCTCAACCTCAAAGCCTTCGTCTCGCAACG
CCTGATCCCCACCATTGACGGCAAGCGCGTTGCCGCCATCGAAATCCTGCTCGGCACACCGATGATTCGCGACCTGATCA
TGAAAGGCGACGTGCACGCCATCAAAGAAATCATGGAAAAGTCCGAAGAACAGGGAATGCAAACCTTCGACAGTCACCTC
TACAAGCTGTATCTGGCCGGGCAAATCTCACTGGCAGAAACCTTGCGCAACGCCGACTCCCCCAGCAACCTCAAGCTCAA
GATCAATCTGTCGGGGAATCTGGCGAAACCACGTCCGACAGCAGCGGCGGCGGCACCTGAAGCCAAATCCACGGCCGCAA
GTGCTGATTTCATGGCGAATTTGTCACTGGAGCCAAAACCAGAGGTGGAACTGACGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(35-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.102

89.796

0.513
  pilU Acinetobacter baylyi ADP1

54.318

91.582

0.497
  pilU Vibrio cholerae strain A1552

49.28

88.52

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.91

96.173

0.403