Detailed information NTG0872518

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RCG00_RS01760
• Genome accession: NZ_CP133217
• Coordinates: 188710..189888 (+)
• Length: 392 a.a.
• NCBI ID: WP_308135375.1
• Uniprot ID: A0AA51R4W2
• Organism: Thiothrix subterranea strain DNT52
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 183710..194888

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RCG00_RS01740 (RCG00_01740)	-	183947..185641 (+)	1695	WP_308135371.1	AAA family ATPase	-
RCG00_RS01745 (RCG00_01745)	rpiA	185707..186363 (-)	657	WP_308135372.1	ribose-5-phosphate isomerase RpiA	-
RCG00_RS01750 (RCG00_01750)	ilvA	186457..187968 (+)	1512	WP_308135373.1	threonine ammonia-lyase, biosynthetic	-
RCG00_RS01755 (RCG00_01755)	-	187950..188657 (-)	708	WP_308135374.1	pseudouridine synthase	-
RCG00_RS01760 (RCG00_01760)	pilU	188710..189888 (+)	1179	WP_308135375.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RCG00_RS01765 (RCG00_01765)	argS	189889..191649 (-)	1761	WP_308135376.1	arginine--tRNA ligase	-
RCG00_RS01770 (RCG00_01770)	-	191748..192482 (+)	735	WP_308135377.1	SprT family zinc-dependent metalloprotease	-
RCG00_RS01775 (RCG00_01775)	yciA	192486..192878 (-)	393	WP_308135378.1	acyl-CoA thioester hydrolase YciA	-
RCG00_RS01780 (RCG00_01780)	-	193093..194661 (+)	1569	WP_308872012.1	DUF1538 family protein	-
RCG00_RS01785 (RCG00_01785)	-	194658..194867 (+)	210	WP_308872013.1	DUF1538 family protein	-

Sequence

Protein

• Length: 392 a.a.
• Molecular weight: 43811.75 Da
• Isoelectric Point: 7.3343

>NTDB_id=872518 RCG00_RS01760 WP_308135375.1 188710..189888(+) (pilU) [Thiothrix subterranea strain DNT52]
MKLKEYLRILAHYDGSDLYLTADLEPKAKFQGKLKAVDKVIMTPEMLKAMAYELMNPEQQQQFEKKPEMNLAISEEGIGR
FRVNIFKQRHKIAMVIRNIKTDIPNADKLGLPQVLKDVIMEKRGLILFVGGTGSGKSTSLAALIDHRNASADGHIITIED
PVEYVHPHKKCIINQREVGVDTDSYEDALKNTLRQAPDVILIGEIRAQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRHQLLMDLSLNLKAFVSQRLIPTVDGKRVAAIEILLGTPMVRDLIMKGDVHAIKETMEKSEEQGMQTFDSHL
YKLYLAGQISLAETLRNADSPSNLKLKINLSGNLNKPRPTAAAPAPEAKPKAADENFMAKLSLQPKPEAELT

Nucleotide

• Length: 1179 bp

>NTDB_id=872518 RCG00_RS01760 WP_308135375.1 188710..189888(+) (pilU) [Thiothrix subterranea strain DNT52]
ATGAAACTCAAAGAATATCTACGCATCCTCGCCCATTACGACGGCTCCGACCTGTATTTAACCGCCGATCTCGAACCCAA
AGCCAAATTCCAAGGCAAGCTCAAAGCCGTCGATAAAGTCATCATGACGCCGGAAATGCTCAAAGCAATGGCTTACGAAT
TGATGAACCCCGAACAGCAGCAACAGTTTGAAAAGAAACCCGAAATGAACCTCGCCATCAGCGAAGAAGGCATCGGACGT
TTCCGCGTCAACATCTTCAAGCAACGCCACAAAATCGCGATGGTGATCCGCAATATCAAAACCGACATCCCCAACGCCGA
CAAACTCGGCCTACCGCAAGTTTTGAAAGACGTGATCATGGAAAAGCGCGGGCTGATCCTGTTCGTCGGCGGCACAGGTT
CGGGTAAATCCACCTCACTGGCGGCACTGATCGACCACCGCAACGCCAGTGCGGACGGGCACATCATCACCATCGAAGAC
CCGGTGGAATACGTGCATCCGCACAAAAAATGTATCATCAACCAGCGCGAGGTTGGCGTAGACACCGACAGCTACGAAGA
CGCGCTCAAAAACACCCTGCGCCAAGCCCCCGACGTGATTTTAATCGGCGAAATCCGCGCCCAAGAAACCATGGAACACG
CCCTCGCCTTCGCCGAAACTGGGCATTTGTGCCTTTCCACCCTGCACGCCAACAACGCCAACCAAGCACTCGACCGCATT
ATCAACTTCTTCCCCGAAGAACGCCGCCACCAATTGCTGATGGATTTATCCCTCAACCTCAAAGCCTTCGTCTCGCAACG
CCTGATCCCAACCGTGGACGGCAAGCGCGTTGCCGCCATCGAAATCCTGCTCGGCACGCCAATGGTGCGCGACCTGATCA
TGAAAGGCGACGTGCACGCCATCAAAGAAACGATGGAGAAGTCCGAAGAACAAGGAATGCAAACCTTCGACAGCCACTTA
TACAAGCTGTATCTGGCGGGGCAAATTTCACTGGCGGAAACCTTGCGCAATGCCGATTCGCCTAGCAATTTGAAGCTGAA
GATTAACTTGTCGGGGAATTTGAATAAGCCGCGTCCGACGGCTGCCGCACCTGCGCCAGAGGCAAAGCCCAAGGCGGCGG
ATGAGAATTTCATGGCGAAATTGTCGTTGCAACCGAAGCCGGAAGCAGAATTGACGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (35-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.044	92.857	0.52
pilU	Acinetobacter baylyi ADP1	54.596	91.582	0.5
pilU	Vibrio cholerae strain A1552	49.284	89.031	0.439
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.623	93.367	0.398