Detailed information NTG0872417

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RCF95_RS03990
• Genome accession: NZ_CP133197
• Coordinates: 673271..674449 (+)
• Length: 392 a.a.
• NCBI ID: WP_308135375.1
• Uniprot ID: A0AA51R4W2
• Organism: Thiothrix subterranea strain DNT53
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 668271..679449

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RCF95_RS03970	-	668510..670203 (+)	1694	Protein_774	AAA family ATPase	-
RCF95_RS03975	rpiA	670269..670924 (-)	656	Protein_775	ribose-5-phosphate isomerase RpiA	-
RCF95_RS03980	ilvA	671018..672529 (+)	1512	WP_308135373.1	threonine ammonia-lyase, biosynthetic	-
RCF95_RS03985	-	672511..673218 (-)	708	WP_308874226.1	pseudouridine synthase	-
RCF95_RS03990	pilU	673271..674449 (+)	1179	WP_308135375.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RCF95_RS03995	argS	674450..676210 (-)	1761	WP_308135376.1	arginine--tRNA ligase	-
RCF95_RS04000	-	676309..677043 (+)	735	WP_308135377.1	SprT family zinc-dependent metalloprotease	-
RCF95_RS04005	yciA	677047..677439 (-)	393	WP_308135378.1	acyl-CoA thioester hydrolase YciA	-
RCF95_RS04010	-	677654..679261 (+)	1608	WP_308874227.1	DUF1538 family protein	-
RCF95_RS04015	-	679218..679427 (+)	210	WP_308872013.1	DUF1538 family protein	-

Sequence

Protein

• Length: 392 a.a.
• Molecular weight: 43811.75 Da
• Isoelectric Point: 7.3343

>NTDB_id=872417 RCF95_RS03990 WP_308135375.1 673271..674449(+) (pilU) [Thiothrix subterranea strain DNT53]
MKLKEYLRILAHYDGSDLYLTADLEPKAKFQGKLKAVDKVIMTPEMLKAMAYELMNPEQQQQFEKKPEMNLAISEEGIGR
FRVNIFKQRHKIAMVIRNIKTDIPNADKLGLPQVLKDVIMEKRGLILFVGGTGSGKSTSLAALIDHRNASADGHIITIED
PVEYVHPHKKCIINQREVGVDTDSYEDALKNTLRQAPDVILIGEIRAQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRHQLLMDLSLNLKAFVSQRLIPTVDGKRVAAIEILLGTPMVRDLIMKGDVHAIKETMEKSEEQGMQTFDSHL
YKLYLAGQISLAETLRNADSPSNLKLKINLSGNLNKPRPTAAAPAPEAKPKAADENFMAKLSLQPKPEAELT

Nucleotide

• Length: 1179 bp

>NTDB_id=872417 RCF95_RS03990 WP_308135375.1 673271..674449(+) (pilU) [Thiothrix subterranea strain DNT53]
ATGAAACTCAAAGAATATCTACGCATCCTCGCCCATTACGACGGCTCCGACCTGTATTTAACCGCCGATCTCGAACCCAA
AGCCAAATTCCAAGGCAAGCTCAAAGCCGTCGATAAAGTCATCATGACGCCGGAAATGCTCAAAGCAATGGCTTACGAAT
TGATGAACCCCGAACAGCAGCAACAGTTTGAAAAGAAACCCGAAATGAACCTCGCCATCAGCGAAGAAGGCATCGGACGT
TTCCGCGTCAACATCTTCAAGCAACGCCACAAAATCGCGATGGTGATCCGCAATATCAAAACCGACATCCCCAACGCCGA
CAAACTCGGCCTACCGCAAGTTTTGAAAGACGTGATCATGGAAAAGCGCGGGCTGATCCTGTTCGTCGGCGGCACAGGTT
CGGGTAAATCCACCTCACTGGCGGCACTGATCGACCACCGCAACGCCAGTGCGGACGGGCACATCATCACCATCGAAGAC
CCGGTGGAATACGTGCATCCGCACAAAAAATGTATCATCAACCAGCGCGAGGTTGGCGTAGACACCGACAGCTACGAAGA
CGCGCTCAAAAACACCCTGCGCCAAGCCCCCGACGTGATTTTAATCGGCGAAATCCGCGCCCAAGAAACCATGGAACACG
CCCTCGCCTTCGCCGAAACTGGGCATTTGTGCCTTTCCACCCTGCACGCCAACAACGCCAACCAAGCACTCGACCGCATT
ATCAACTTCTTCCCCGAAGAACGCCGCCACCAATTGCTGATGGATTTATCCCTCAACCTCAAAGCCTTCGTCTCGCAACG
CCTGATCCCAACCGTGGACGGCAAGCGCGTTGCCGCCATCGAAATCCTGCTCGGCACGCCAATGGTGCGCGACCTGATCA
TGAAAGGCGACGTGCACGCCATCAAAGAAACGATGGAGAAGTCCGAAGAACAAGGAATGCAAACCTTCGACAGCCACTTA
TACAAGCTGTATCTGGCGGGGCAAATTTCACTGGCGGAAACCTTGCGCAATGCCGATTCGCCTAGCAATTTGAAGCTGAA
GATTAACTTGTCGGGGAATTTGAATAAGCCGCGTCCGACGGCTGCCGCACCTGCGCCAGAGGCAAAGCCCAAGGCGGCGG
ATGAGAATTTCATGGCGAAATTGTCGTTGCAACCGAAGCCGGAAGCAGAATTGACGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (35-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.044	92.857	0.52
pilU	Acinetobacter baylyi ADP1	54.596	91.582	0.5
pilU	Vibrio cholerae strain A1552	49.284	89.031	0.439
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.623	93.367	0.398